临床实践中CD34表达细胞的分析

Analysis of CD34-expressing cells in clinical practice.

作者信息

Serke S, Beyer J, Rick O, Schwella N, Meyer O, Hildebrandt M, Huhn D

机构信息

Department of Haematology-Oncology, Campus Virchow Hospital Charite, Humboldt University, Berlin, Germany.

出版信息

Vox Sang. 1998;74 Suppl 2:469-75. doi: 10.1111/j.1423-0410.1998.tb05459.x.

DOI:10.1111/j.1423-0410.1998.tb05459.x

PMID:9704484

Abstract

The flow cytometric determination of haemopoietic cells defined as CD34-expressing cells has greatly added to the improvement of the management of harvesting circulating haemopoietic cells for subsequent autologous reinfusion in the setting of high-dose chemo/(radio)-therapy. Additionally, this flow cytometric determination has replaced, in some institutions, the in-vitro culture test for CFU-GM as the measure to estimate the haemopoietic potential of the cells to be reinfused/transplanted.

摘要

通过流式细胞术测定定义为表达CD34的造血细胞，极大地促进了采集循环造血细胞管理的改进，以便在大剂量化疗/（放疗）情况下随后进行自体回输。此外，在一些机构中，这种流式细胞术测定已取代了CFU-GM的体外培养试验，作为评估待回输/移植细胞造血潜力的方法。