Pang M, Abe T, Fujihara T, Mori S, Tsuzaka K, Amano K, Koide J, Takeuchi T
Saitama Medical School, Kawagoe, Japan.
Arthritis Rheum. 1998 Aug;41(8):1456-63. doi: 10.1002/1529-0131(199808)41:8<1456::AID-ART16>3.0.CO;2-0.
To determine the possible role of a novel integrin, alphaEbeta7, in the pathogenesis of systemic lupus erythematosus (SLE).
Expression of alphaEbeta7 was examined on peripheral blood lymphocytes (PBL) from normal subjects (n = 25) and patients with SLE (n = 31), primary Sjogren's syndrome (n = 7), or polymyositis/dermatomyositis (n = 8) by cytofluorometry and/or immunoprecipitation. Adhesion of alphaEbeta7+ T cells to HSG epithelial cells was investigated using a confocal image analyzer.
After phytohemagglutinin stimulation, expression of alphaEbeta7 on PBL, especially on CD8+ T cells, was significantly higher in SLE patients than in normal subjects (P<0.01). Elevated alphaEbeta7 expression was associated with the presence of oral ulcers or serositis (P<0.05). Activated SLE T cells with enhanced alphaEbeta7 expression strongly adhered to HSG; this adhesion was partially blocked by anti-alphaEbeta7.
Expression and adhesion of alphaEbeta7 on activated PBL was significantly increased in patients with SLE with epithelial involvement. This suggests a role of this novel integrin in tissue-specific retention of activated PBL, due to increased alphaEbeta7-E-cadherin interaction, which may contribute to epithelial inflammation.
确定一种新型整合素αEβ7在系统性红斑狼疮（SLE）发病机制中的可能作用。
通过细胞荧光测定法和/或免疫沉淀法检测25名正常受试者、31名SLE患者、7名原发性干燥综合征患者或8名多发性肌炎/皮肌炎患者外周血淋巴细胞（PBL）上αEβ7的表达。使用共聚焦图像分析仪研究αEβ7 + T细胞与HSG上皮细胞的黏附情况。
在植物血凝素刺激后，SLE患者PBL上αEβ7的表达，尤其是CD8 + T细胞上的表达，显著高于正常受试者（P < 0.01）。αEβ7表达升高与口腔溃疡或浆膜炎的存在相关（P < 0.05）。αEβ7表达增强的活化SLE T细胞与HSG强烈黏附；这种黏附被抗αEβ7部分阻断。
在有上皮受累的SLE患者中，活化PBL上αEβ7的表达和黏附显著增加。这表明这种新型整合素通过增加αEβ7-E-钙黏蛋白相互作用，在活化PBL的组织特异性滞留中发挥作用，这可能导致上皮炎症。
