Elewaut D, De Keyser F, Van Den Bosch F, Lazarovits A I, De Vos M, Cuvelier C, Verbruggen G, Mielants H, Veys E M
Department of Rheumatology, University Hospital Ghent, Belgium.
J Rheumatol. 1998 Oct;25(10):1932-7.
To compare the expression of adhesion molecules on synovial T cells from patients with early spondyloarthropathy (SpA) and rheumatoid arthritis (RA), with special reference to the beta7 integrins alpha4beta7 and alphaEbeta7 in view of their intimate association with intestinal tissue.
Twenty-five synovial cell lines were generated by interleukin 2 (IL-2) expansion from synovial biopsies of patients with early SpA and RA, obtained from macroscopically inflamed synovial tissue by needle arthroscopy, and subsequently characterized by flow cytometry for CD3, CD4, CD8, L-selectin, CD11a, CD31, CD44, and alpha4beta7 and alphaEbeta7 integrin.
In SpA, the beta7 integrin expression was increased, compared to RA. Furthermore, an inverse relation between alpha4beta7 and alphaEbeta7 was present in SpA (r = -0.75, p < 0.02), as on many mucosal T cells. In contrast, an opposite correlation was noted in RA (r = +0.84, p < 0.01), as similarly described on a subset of circulating T cells.
Increased expression of beta7 integrins was noted on synovial T cell lines from SpA compared to RA, with discriminative correlations between alpha4beta7 and alphaEbeta7. This suggests a different origin of the synovial T cells in these diseases.
比较早期脊柱关节炎（SpA）和类风湿关节炎（RA）患者滑膜T细胞上黏附分子的表达情况，鉴于β7整合素α4β7和αEβ7与肠道组织密切相关，特别关注这两种整合素。
通过白细胞介素2（IL-2）扩增，从早期SpA和RA患者的滑膜活检组织中生成25个滑膜细胞系，这些活检组织通过针式关节镜从宏观上发炎的滑膜组织获取，随后通过流式细胞术对CD3、CD4、CD8、L-选择素、CD11a、CD31、CD44以及α4β7和αEβ7整合素进行表征。
与RA相比，SpA中β7整合素表达增加。此外，与许多黏膜T细胞一样，SpA中α4β7和αEβ7之间存在负相关（r = -0.75，p < 0.02）。相反，在RA中观察到相反的相关性（r = +0.84，p < 0.01），这与在一部分循环T细胞上的描述相似。
与RA相比，SpA滑膜T细胞系上β7整合素表达增加，α4β7和αEβ7之间存在区别性相关性。这表明这些疾病中滑膜T细胞的起源不同。
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