感染母亲及其婴儿中HIV-1 E亚型的V3序列多样性。

V3 sequence diversity of HIV-1 subtype E in infected mothers and their infants.

作者信息

Sutthent R, Foongladda S, Chearskul S, Wanprapa N, Likanonskul S, Kositanont U, Riengrojpitak S, Sahaphong S, Wasi C

机构信息

Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

出版信息

J Acquir Immune Defic Syndr Hum Retrovirol. 1998 Aug 1;18(4):323-31. doi: 10.1097/00042560-199808010-00003.

DOI:10.1097/00042560-199808010-00003

PMID:9704937

Abstract

To elucidate genetic characteristics of HIV-1 subtype E involved in vertical transmission, V3 regions of HIV-1 subtype E isolated from 17 infected mothers (M1-M17) and their infants (I1-I17) at 1 month after birth were sequenced after cloned into pCRII vectors. At least three clones of each sample were collected. All mothers were asymptomatic and had been infected through a heterosexual route. Nine infants (I9-I17) showed mild symptomatic and immunosuppression within the first year of life. The interpatient nucleotide distance of mothers and infants in this group (0.065+/-0.008) were of greater diversity than those of a nonimmunosuppression group (0.039+/-0.006) by a significant amount (Fischer's exact test, p = .003). The substitution with asparagine (N) at threonine (T) at position 13 and aspartic acid (D) at position 29 of the V3 sequence were significantly associated with nonimmunosuppression in the first year of life (F-test, p = 0.003). Either a single or multiple viral variants could transmit from mothers to their infants.

摘要

为阐明参与垂直传播的HIV-1 E亚型的基因特征，将从17名感染母亲（M1-M17）及其出生后1个月的婴儿（I1-I17）中分离出的HIV-1 E亚型的V3区克隆到pCRII载体中后进行测序。每个样本至少收集三个克隆。所有母亲均无症状，且通过异性传播途径感染。9名婴儿（I9-I17）在生命的第一年内出现轻度症状和免疫抑制。该组中母亲和婴儿的患者间核苷酸距离（0.065±0.008）比非免疫抑制组（0.039±0.006）具有更大的多样性，差异具有统计学意义（费舍尔精确检验，p = 0.003）。V3序列第13位苏氨酸（T）被天冬酰胺（N）取代以及第29位天冬氨酸（D）被取代与生命第一年内的非免疫抑制显著相关（F检验，p = 0.003）。单个或多个病毒变体均可从母亲传播给婴儿。