Smit N P, Kolb R M, Lentjes E G, Noz K C, van der Meulen H, Koerten H K, Vermeer B J, Pavel S
Department of Dermatology, Leiden University Medical Centre, The Netherlands.
Arch Dermatol Res. 1998 Jun;290(6):342-9. doi: 10.1007/s004030050315.
In many laboratories, culturing skin melanocytes has become a routine research activity. However, recent investigations have revealed that the quality and quantity of the pigment formed in the cultured cells may differ significantly from those of the original skin pigment cells. To shed more light on this issue, we examined the influence of different culture media on pigment production. We showed that there were notable passage-to-passage variations in the synthesis of melanin. This was particularly true for phaeomelanin. It is therefore advisable to analyse the melanin in the cells before the start of experiments. In spite of the variations, basic differences in the pigmentation pattern between melanocytes isolated from light-skinned and dark-skinned individuals remained preserved in the corresponding cultures as observed by electron microscopy. Also, the total melanin content was higher in a skin type VI melanocyte culture than in skin type I and II melanocyte cultures. In contrast to total melanin, the phaeomelanin concentration of skin type VI cells was similar to that of the skin type I melanocytes. With higher L-tyrosine concentrations in the medium, as well as increased eumelanin synthesis, phaeomelanogenesis was also stimulated in all cultures tested. This stimulation was particularly prominent in skin type I melanocytes. Our preliminary experiments also showed that a melanocyte culture from atypical naevus cells exhibited a similar preference for phaeomelanogenesis when pigmentation was stimulated.
在许多实验室中，培养皮肤黑素细胞已成为一项常规研究活动。然而，最近的研究表明，培养细胞中形成的色素的质量和数量可能与原始皮肤色素细胞的有显著差异。为了更清楚地了解这个问题，我们研究了不同培养基对色素生成的影响。我们发现黑色素合成存在明显的传代差异。对于褐黑素来说尤其如此。因此，在实验开始前分析细胞中的黑色素是明智的。尽管存在差异，但通过电子显微镜观察，在相应培养物中，从浅色皮肤和深色皮肤个体分离的黑素细胞之间色素沉着模式的基本差异仍然存在。此外，VI型皮肤黑素细胞培养物中的总黑色素含量高于I型和II型皮肤黑素细胞培养物。与总黑色素不同，VI型皮肤细胞的褐黑素浓度与I型皮肤黑素细胞的相似。随着培养基中L-酪氨酸浓度的升高以及真黑素合成的增加，所有测试培养物中的褐黑素生成也受到刺激。这种刺激在I型皮肤黑素细胞中尤为明显。我们的初步实验还表明，当色素沉着受到刺激时，来自非典型痣细胞的黑素细胞培养物对褐黑素生成表现出类似的偏好。
