Essawi T, Na'was T, Hawwari A, Wadi S, Doudin A, Fattom A I
Department of Biology and Biochemistry, Birzeit University, Palestine.
Trop Med Int Health. 1998 Jul;3(7):576-83. doi: 10.1046/j.1365-3156.1998.00265.x.
Staphylococcus aureus is a major cause of nosocomial infections and a risk in patients who have either undergone surgery or are on haemodialysis. The S. aureus infections in patients admitted to the clinical departments of Al-Makased Charitable Hospital in Jerusalem during a period of one year were investigated. Isolates included were from blood, surgical wounds, or other nonsuperficial sites. Of 63 isolates available for analysis, 46 (73.0%) expressed type 8 capsular polysaccharide; 13 (20.7%), type 5 capsular polysaccharide; only 4 isolates (6.3%) did not express type 5 or type 8 antibodies. The strains fitted in 7 different antibiogram types, with the type showing resistance only to penicillin and ampicillin prevalent in 34 out of 63 isolates (54.0%). Of the 12 methicillin-resistant S. aureus (MRSA) isolates (19.1%), 8(66.7%) possessed the type 8 capsule and 4(33.7%) the type 5 capsule. Pulsed-field gel electrophoresis of all isolates with the restriction-endonuclease enzymes Sma I revealed 34 patterns demonstrating that no single methicillin-sensitive S. aureus strain was endemic in the hospital. However, all MRSA isolates with a type 8 capsule showed identical PFGE patterns using the 2 restriction-endonuclease enzymes Sma I and SST II. Moreover, type 5 isolates showed identical patterns (one isolate differed from the rest with one band only). These data suggest and confirm the clonality of type 5 and type 8 MRSA isolates. Analysing the results of the capsular and antibiogram typing schemes in conjunction proved useful and suggested that such an analysis can be employed as a helpful epidemiological tool in hospitals with limited resources.
金黄色葡萄球菌是医院感染的主要病因，对于接受过手术或进行血液透析的患者构成风险。对耶路撒冷Al-Makased慈善医院临床科室收治的患者在一年期间的金黄色葡萄球菌感染情况进行了调查。纳入分析的菌株分离自血液、手术伤口或其他非浅表部位。在可供分析的63株菌株中，46株（73.0%）表达8型荚膜多糖；13株（20.7%）表达5型荚膜多糖；仅有4株（6.3%）不表达5型或8型抗体。这些菌株分为7种不同的抗菌谱类型，其中仅对青霉素和氨苄西林耐药的类型在63株菌株中有34株（54.0%）较为常见。在12株耐甲氧西林金黄色葡萄球菌（MRSA）菌株（19.1%）中，8株（66.7%）具有8型荚膜，4株（33.7%）具有5型荚膜。用限制性内切酶Sma I对所有菌株进行脉冲场凝胶电泳显示有34种图谱，表明该医院没有单一的甲氧西林敏感金黄色葡萄球菌菌株为地方流行株。然而，所有具有8型荚膜的MRSA菌株在用限制性内切酶Sma I和SST II进行分析时显示出相同的PFGE图谱。此外，5型菌株显示出相同的图谱（一株菌株仅一条带与其他菌株不同）。这些数据提示并证实了5型和8型MRSA菌株的克隆性。结合分析荚膜分型和抗菌谱分型方案的结果被证明是有用的，表明这种分析可作为资源有限医院中一种有用的流行病学工具。
