通过扩增16S - 23S核糖体DNA间隔区鉴别结核分枝杆菌和鸟分枝杆菌
Differentiation between Mycobacterium tuberculosis and Mycobacterium avium by amplification of the 16S-23S ribosomal DNA spacer.
作者信息
Sansila A, Hongmanee P, Chuchottaworn C, Rienthong S, Rienthong D, Palittapongarnpim P
机构信息
Department of Pathology, Faculty of Veterinary Medicine, Khonkaen University, Bangkok, Thailand.
出版信息
J Clin Microbiol. 1998 Sep;36(9):2399-403. doi: 10.1128/JCM.36.9.2399-2403.1998.
Abstract
Differentiation between Mycobacterium tuberculosis and M. avium is helpful for the treatment of disseminated mycobacterial infection in AIDS patients. This can traditionally be done by time-consuming biochemical tests or with Accuprobe. Previously, PCR restriction enzyme analysis (PCR-REA) of the 16S-23S rRNA gene spacer was shown to be able to identify a limited number of strains of Mycobacterium. In this study the method was improved by using more specific primers and was tested with 50 clinical isolates of M. tuberculosis and 65 clinical isolates of M. avium complex. Probes specific to the spacers of M. tuberculosis and M. avium were also tested. Both M. tuberculosis and M. avium could be reliably identified either by PCR-REA or by PCR-hybridization, with the results completely agreeing with those obtained by biochemical tests and with the Accuprobe, respectively. The method may therefore be useful as an alternative in-house method for identification of the bacteria.
摘要
区分结核分枝杆菌和鸟分枝杆菌有助于治疗艾滋病患者的播散性分枝杆菌感染。传统上，这可以通过耗时的生化试验或使用基因探针来完成。此前，16S - 23S rRNA基因间隔区的PCR限制性酶切分析（PCR - REA）已被证明能够鉴定有限数量的分枝杆菌菌株。在本研究中，通过使用更特异的引物改进了该方法，并对50株结核分枝杆菌临床分离株和65株鸟分枝杆菌复合群临床分离株进行了测试。还测试了针对结核分枝杆菌和鸟分枝杆菌间隔区的探针。结核分枝杆菌和鸟分枝杆菌均可通过PCR - REA或PCR杂交可靠鉴定，结果分别与生化试验和基因探针法完全一致。因此，该方法可作为一种替代的实验室内部细菌鉴定方法。
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