van der Giessen J W, Haring R M, van der Zeijst B A
Department of Bacteriology, School of Veterinary Medicine, Utrecht University, The Netherlands.
Microbiology (Reading). 1994 May;140 ( Pt 5):1103-8. doi: 10.1099/13500872-140-5-1103.
The 23S rDNA sequences of Mycobacterium paratuberculosis, M. avium and M. phlei and the sequences of the spacer regions between the 16S and 23S rRNA genes were determined. The overall 23S rDNA sequence identity between M. paratuberculosis and M. avium was 99.7% (nine mismatches), showing the very close relatedness of these mycobacteria. Evolutionary distances between the five known mycobacterial 23S rRNA/rDNA sequences and those of other Gram-positive G + C-rich bacteria were determined. The 23S rDNA sequences of mycobacteria showed two inserted regions compared to the other bacteria. A mycobacterial unique region contained one mismatch between M. paratuberculosis and M. avium. An Actinomycetales-specific insertion, consisting of 111 nucleotides, was completely identical for M. paratuberculosis and M. avium. The sequence of the intergenic spacer region between 16S and 23S rDNA had a length of 278 bp for M. paratuberculosis and M. avium with only two mismatches. The spacer region of the fast-growing M. phlei was 85 bp longer. No tRNA-encoding region was found in the spacer region.
测定了副结核分枝杆菌、鸟分枝杆菌和草分枝杆菌的23S rDNA序列以及16S和23S rRNA基因之间间隔区的序列。副结核分枝杆菌和鸟分枝杆菌之间23S rDNA序列的总体同一性为99.7%（九个错配），表明这些分枝杆菌之间有非常密切的亲缘关系。确定了五个已知分枝杆菌23S rRNA/rDNA序列与其他革兰氏阳性富含G+C细菌的进化距离。与其他细菌相比，分枝杆菌的23S rDNA序列显示出两个插入区域。一个分枝杆菌独特区域在副结核分枝杆菌和鸟分枝杆菌之间包含一个错配。一个由111个核苷酸组成的放线菌目特异性插入序列在副结核分枝杆菌和鸟分枝杆菌中完全相同。副结核分枝杆菌和鸟分枝杆菌的16S和23S rDNA之间的基因间隔区序列长度为278 bp，只有两个错配。快速生长的草分枝杆菌的间隔区长85 bp。在间隔区未发现编码tRNA的区域。
