Realini L, De Ridder K, Palomino J, Hirschel B, Portaels F
Mycobacteriology Unit, Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium.
J Clin Microbiol. 1998 Sep;36(9):2565-70. doi: 10.1128/JCM.36.9.2565-2570.1998.
Our studies show that microaerophilic conditions promote the growth of Mycobacterium genavense in semisolid medium. The growth of M. genavense at 2.5 or 5% oxygen was superior to that obtained at 21% oxygen in BACTEC primary cultures (Middlebrook 7H12, pH 6.0, without additives). By using nondecontaminated specimens, it was possible to detect growth with very small inocula (25 bacilli/ml) of 12 different M. genavense strains (from nude mice) within 6 weeks of incubation under low oxygen tension; conversely, with 21% oxygen, no growth of 8 of 12 (66.7%) M. genavense strains was detected (growth index, <10). The same beneficial effect of 2.5 or 5% oxygen was observed in primary cultures of a decontaminated clinical specimen. Low oxygen tension (2.5 or 5%) is recommended for the primary isolation of M. genavense. Microaerophilic cultivation of other atypical mycobacteria, especially slow-growing (e.g., Mycobacterium avium) and difficult-to-grow (e.g., Mycobacterium ulcerans) species, is discussed.
我们的研究表明，微需氧条件可促进日内瓦分枝杆菌在半固体培养基中的生长。在BACTEC初代培养物（Middlebrook 7H12，pH 6.0，无添加剂）中，日内瓦分枝杆菌在2.5%或5%氧气浓度下的生长优于在21%氧气浓度下的生长。使用未去污的标本，在低氧张力下孵育6周内，能够检测到12种不同的日内瓦分枝杆菌菌株（来自裸鼠）的极少量接种物（25个杆菌/毫升）的生长；相反，在21%氧气浓度下，12种日内瓦分枝杆菌菌株中有8种（66.7%）未检测到生长（生长指数<10）。在一份去污后的临床标本的初代培养物中也观察到了2.5%或5%氧气浓度的相同有益效果。建议采用低氧张力（2.5%或5%）进行日内瓦分枝杆菌的初代分离。本文还讨论了其他非典型分枝杆菌的微需氧培养，尤其是生长缓慢的（如鸟分枝杆菌）和难以生长的（如溃疡分枝杆菌）菌种。
