McCarter Y S, Ratkiewicz I N, Robinson A
Division of Microbiology, Department of Pathology and Laboratory Medicine, Hartford Hospital, Hartford, Connecticut 06102-5037, USA.
J Clin Microbiol. 1998 Sep;36(9):2769-71. doi: 10.1128/JCM.36.9.2769-2771.1998.
Serpentine cord formation in BACTEC 12B medium was evaluated as a rapid method for the presumptive identification of M. tuberculosis complex. Kinyoun acid-fast stained smears were prepared from 666 positive BACTEC 12B bottles and examined for the presence or absence of serpentine cording. Cord formation had a sensitivity, specificity, positive predictive value, and negative predictive value of 89.2, 99.2, 98.5, and 94.2%, respectively. The evaluation of the presence of cord formation in BACTEC 12B medium is reliable and permits the rapid presumptive reporting of M. tuberculosis.
评估了BACTEC 12B培养基中的蛇形索状形成作为结核分枝杆菌复合群初步鉴定的快速方法。从666个BACTEC 12B阳性培养瓶中制备金胺-酚抗酸染色涂片,检查是否存在蛇形索状。索状形成的敏感性、特异性、阳性预测值和阴性预测值分别为89.2%、99.2%、98.5%和94.2%。评估BACTEC 12B培养基中索状形成的存在是可靠的,并且可以快速初步报告结核分枝杆菌。