在BACTEC培养基中形成索状是一种可靠、快速的初步鉴定结核分枝杆菌复合群的方法。
Cord formation in BACTEC medium is a reliable, rapid method for presumptive identification of Mycobacterium tuberculosis complex.
作者信息
McCarter Y S, Ratkiewicz I N, Robinson A
机构信息
Division of Microbiology, Department of Pathology and Laboratory Medicine, Hartford Hospital, Hartford, Connecticut 06102-5037, USA.
出版信息
J Clin Microbiol. 1998 Sep;36(9):2769-71. doi: 10.1128/JCM.36.9.2769-2771.1998.
Abstract
Serpentine cord formation in BACTEC 12B medium was evaluated as a rapid method for the presumptive identification of M. tuberculosis complex. Kinyoun acid-fast stained smears were prepared from 666 positive BACTEC 12B bottles and examined for the presence or absence of serpentine cording. Cord formation had a sensitivity, specificity, positive predictive value, and negative predictive value of 89.2, 99.2, 98.5, and 94.2%, respectively. The evaluation of the presence of cord formation in BACTEC 12B medium is reliable and permits the rapid presumptive reporting of M. tuberculosis.
摘要
评估了BACTEC 12B培养基中的蛇形索状形成作为结核分枝杆菌复合群初步鉴定的快速方法。从666个BACTEC 12B阳性培养瓶中制备金胺-酚抗酸染色涂片，检查是否存在蛇形索状。索状形成的敏感性、特异性、阳性预测值和阴性预测值分别为89.2%、99.2%、98.5%和94.2%。评估BACTEC 12B培养基中索状形成的存在是可靠的，并且可以快速初步报告结核分枝杆菌。
相似文献
1
Cord formation in BACTEC medium is a reliable, rapid method for presumptive identification of Mycobacterium tuberculosis complex.在BACTEC培养基中形成索状是一种可靠、快速的初步鉴定结核分枝杆菌复合群的方法。
J Clin Microbiol. 1998 Sep;36(9):2769-71. doi: 10.1128/JCM.36.9.2769-2771.1998.
2
Selective utilization of DNA probes for identification of Mycobacterium species on the basis of cord formation in primary BACTEC 12B cultures.基于初代BACTEC 12B培养物中的索状形成，选择性利用DNA探针鉴定分枝杆菌菌种。
J Clin Microbiol. 1995 Jun;33(6):1548-50. doi: 10.1128/jcm.33.6.1548-1550.1995.
3
Reliability of cord formation in BACTEC 12B/13A media for presumptive identification of Mycobacterium tuberculosis complex in laboratories with a high prevalence of Mycobacterium tuberculosis.在结核分枝杆菌高流行率实验室中，使用BACTEC 12B/13A培养基进行结核分枝杆菌复合群初步鉴定时索状形成的可靠性。
Eur J Clin Microbiol Infect Dis. 2002 Apr;21(4):314-7. doi: 10.1007/s10096-002-0701-9. Epub 2002 Mar 16.
4
Cord formation in BACTEC(TM) medium aids rapid identification of Mycobacterium tuberculosis complex.BACTEC（商标）培养基中的索状形成有助于快速鉴定结核分枝杆菌复合群。
Int J Tuberc Lung Dis. 2015 Jun;19(6):709-10. doi: 10.5588/ijtld.14.0586.
5
Cord formation in BACTEC 7H12 medium for rapid, presumptive identification of Mycobacterium tuberculosis complex.在BACTEC 7H12培养基中形成索状结构用于快速初步鉴定结核分枝杆菌复合群。
J Clin Microbiol. 1990 Jun;28(6):1451-3. doi: 10.1128/jcm.28.6.1451-1453.1990.
6
7
Presumptive identification of Mycobacterium tuberculosis complex based on cord formation in BACTEC MGIT 960 medium.基于BACTEC MGIT 960培养基中索状形成对结核分枝杆菌复合群进行初步鉴定。
Indian J Med Microbiol. 2012 Apr-Jun;30(2):218-21. doi: 10.4103/0255-0857.96697.
8
Assessment of morphology for rapid presumptive identification of Mycobacterium tuberculosis and Mycobacterium kansasii.用于快速初步鉴定结核分枝杆菌和堪萨斯分枝杆菌的形态学评估。
J Clin Microbiol. 2000 Apr;38(4):1426-9. doi: 10.1128/JCM.38.4.1426-1429.2000.
9
10
Enhanced Amplified Mycobacterium Tuberculosis Direct Test for detection of Mycobacterium tuberculosis complex in positive BACTEC 12B broth cultures of respiratory specimens.用于在呼吸道标本的阳性BACTEC 12B肉汤培养物中检测结核分枝杆菌复合群的增强型扩增结核分枝杆菌直接试验。
J Clin Microbiol. 1999 Jun;37(6):2099-101. doi: 10.1128/JCM.37.6.2099-2101.1999.
引用本文的文献
1
Drug Susceptibility and Mutation Profiles in Mycobacterium tuberculosis Isolates from a Tertiary Care Hospital in Kerala, India.印度喀拉拉邦一家三级保健医院分离的结核分枝杆菌的药物敏感性和突变特征。
Am J Trop Med Hyg. 2024 May 21;111(1):161-167. doi: 10.4269/ajtmh.24-0042. Print 2024 Jul 3.
2
Mechanopathology of biofilm-like Mycobacterium tuberculosis cords.生物膜样分枝杆菌索的机械病理学。
Cell. 2023 Nov 9;186(23):5135-5150.e28. doi: 10.1016/j.cell.2023.09.016. Epub 2023 Oct 20.
3
Identification of mycobacteria species by molecular methods.采用分子方法鉴定分枝杆菌菌种。
Int Wound J. 2020 Apr;17(2):245-250. doi: 10.1111/iwj.13238. Epub 2019 Dec 20.
4
Practical Guidance for Clinical Microbiology Laboratories: Mycobacteria.临床微生物学实验室实用指南：分枝杆菌。
Clin Microbiol Rev. 2018 Jan 31;31(2). doi: 10.1128/CMR.00038-17. Print 2018 Apr.
5
Deletion of a dehydratase important for intracellular growth and cording renders rough Mycobacterium abscessus avirulent.缺失对细胞内生长和索状形成很重要的一种脱水酶会使粗糙型脓肿分枝杆菌失去毒性。
Proc Natl Acad Sci U S A. 2016 Jul 19;113(29):E4228-37. doi: 10.1073/pnas.1605477113. Epub 2016 Jul 6.
6
Novel approach for improving sensitivity of microscopic detection of acid-fast bacilli (AFB) by use of the ReaSLR method.利用 ReaSLR 方法提高抗酸杆菌（AFB）显微镜检测灵敏度的新方法。
J Clin Microbiol. 2013 Nov;51(11):3597-601. doi: 10.1128/JCM.01570-13. Epub 2013 Aug 21.
7
Demonstration of cord formation by rough Mycobacterium abscessus variants: implications for the clinical microbiology laboratory.粗糙型脓肿分枝杆菌变异体形成索状结构的示教：对临床微生物学实验室的影响。
J Clin Microbiol. 2011 Jun;49(6):2293-5. doi: 10.1128/JCM.02322-10. Epub 2011 Apr 13.
8
Evaluation of the rapid MGIT TBc identification test for culture confirmation of Mycobacterium tuberculosis complex strain detection.评价快速 MGIT TBc 鉴定试验对结核分枝杆菌复合群菌株检测的培养确认。
J Clin Microbiol. 2011 Mar;49(3):802-7. doi: 10.1128/JCM.02243-10. Epub 2010 Dec 29.
9
Diagnostic accuracy of the microscopic observation drug susceptibility assay: a pilot study from India.微量药物敏感性检测法的诊断准确性：来自印度的一项初步研究。
Int J Tuberc Lung Dis. 2010 Apr;14(4):482-8.
10
Microscopic cords, a virulence-related characteristic of Mycobacterium tuberculosis, are also present in nonpathogenic mycobacteria.微观索状物是结核分枝杆菌的一种与毒力相关的特征，也存在于非致病性分枝杆菌中。
J Bacteriol. 2010 Apr;192(7):1751-60. doi: 10.1128/JB.01485-09. Epub 2010 Jan 22.
本文引用的文献
1
VIRULENCE AND MORPHOLOGICAL CHARACTERISTICS OF MAMMALIAN TUBERCLE BACILLI.哺乳动物结核分枝杆菌的毒力和形态特征。
J Exp Med. 1947 Jul 31;86(2):175-84. doi: 10.1084/jem.86.2.175.
2
Guidelines for preventing the transmission of Mycobacterium tuberculosis in health-care facilities, 1994. Centers for Disease Control and Prevention.《医疗机构预防结核分枝杆菌传播指南》，1994年。疾病控制与预防中心。
MMWR Recomm Rep. 1994 Oct 28;43(RR-13):1-132.
3
The resurgence of tuberculosis: is your laboratory ready?结核病的卷土重来：你的实验室准备好了吗？
J Clin Microbiol. 1993 Apr;31(4):767-70. doi: 10.1128/jcm.31.4.767-770.1993.
4
Reliability of cord formation in BACTEC media for presumptive identification of mycobacteria.用于分枝杆菌初步鉴定的BACTEC培养基中索状形成的可靠性。
J Clin Microbiol. 1993 Sep;31(9):2533-4. doi: 10.1128/jcm.31.9.2533-2534.1993.
5
The laboratory diagnosis of mycobacterial diseases. Challenges and common sense.分枝杆菌病的实验室诊断。挑战与常识。
Clin Lab Med. 1995 Jun;15(2):279-306.
6
Selective utilization of DNA probes for identification of Mycobacterium species on the basis of cord formation in primary BACTEC 12B cultures.基于初代BACTEC 12B培养物中的索状形成，选择性利用DNA探针鉴定分枝杆菌菌种。
J Clin Microbiol. 1995 Jun;33(6):1548-50. doi: 10.1128/jcm.33.6.1548-1550.1995.
7
Identification of Mycobacterium tuberculosis and M. avium complex directly from smear-positive sputum specimens and BACTEC 12B cultures by high-performance liquid chromatography with fluorescence detection and computer-driven pattern recognition models.通过荧光检测高效液相色谱法和计算机驱动模式识别模型直接从涂片阳性痰标本和BACTEC 12B培养物中鉴定结核分枝杆菌和鸟分枝杆菌复合群。
J Clin Microbiol. 1995 May;33(5):1270-7. doi: 10.1128/jcm.33.5.1270-1277.1995.
8
Application of new technology to the detection, identification, and antimicrobial susceptibility testing of mycobacteria.
Am J Clin Pathol. 1994 Mar;101(3):329-37. doi: 10.1093/ajcp/101.3.329.
9
Detection and identification of Mycobacterium directly from BACTEC bottles by using a DNA-rRNA probe.通过使用DNA-rRNA探针直接从BACTEC瓶中检测和鉴定分枝杆菌。
Diagn Microbiol Infect Dis. 1993 Oct;17(3):203-7. doi: 10.1016/0732-8893(93)90097-q.
10
Cord formation in BACTEC 7H12 medium for rapid, presumptive identification of Mycobacterium tuberculosis complex.在BACTEC 7H12培养基中形成索状结构用于快速初步鉴定结核分枝杆菌复合群。
J Clin Microbiol. 1990 Jun;28(6):1451-3. doi: 10.1128/jcm.28.6.1451-1453.1990.
Zotero 插件