在结核分枝杆菌高流行率实验室中，使用BACTEC 12B/13A培养基进行结核分枝杆菌复合群初步鉴定时索状形成的可靠性。

Reliability of cord formation in BACTEC 12B/13A media for presumptive identification of Mycobacterium tuberculosis complex in laboratories with a high prevalence of Mycobacterium tuberculosis.

作者信息

Köksalan O K, Aydin M D, Eraslan S, Bekiroglu N

机构信息

Department of Genetics, Institute for Medical Experimental Research (DETAE), Istanbul University, Istanbul, Turkey.

出版信息

Eur J Clin Microbiol Infect Dis. 2002 Apr;21(4):314-7. doi: 10.1007/s10096-002-0701-9. Epub 2002 Mar 16.

DOI:10.1007/s10096-002-0701-9

PMID:12072946

Abstract

A total of 1208 positive BACTEC vials were examined for the presence or absence of serpentine cording. A very high (92.9%) rate of laboratory prevalence was obtained for Mycobacterium tuberculosis complex. The sensitivity, specificity, positive and negative predictive values of this test were 92.7%, 95.3%, 99.6% and 50.0%, respectively. It was concluded that testing cord formation in laboratories that have a high prevalence of Mycobacterium tuberculosis complex is an exceptionally reliable method for preliminary reporting of cording-positive cases; however, for cording-negative cases, preliminary reports based solely on cord formation are not reliable. It was also observed that the length of the incubation period has a significant effect on cord formation. Incubation periods of 4 days or less are not sufficient to determine noncording in smears prepared from positive BACTEC vials.

摘要

共对1208个BACTEC阳性培养瓶进行了蛇形索状形成检测。结核分枝杆菌复合群的实验室检出率非常高（92.9%）。该检测的敏感性、特异性、阳性预测值和阴性预测值分别为92.7%、95.3%、99.6%和50.0%。得出的结论是，在结核分枝杆菌复合群患病率高的实验室中检测索状形成是初步报告索状阳性病例的一种极其可靠的方法；然而，对于索状阴性病例，仅基于索状形成的初步报告并不可靠。还观察到潜伏期的长短对索状形成有显著影响。4天或更短的潜伏期不足以确定从BACTEC阳性培养瓶制备的涂片无索状形成。