Ozawa T, Wang J, Hu L J, Lamborn K R, Bollen A W, Deen D F
Department of Neurological Surgery, School of Medicine, University of California, San Francisco 94143-0520, USA.
In Vivo. 1998 Jul-Aug;12(4):369-74.
BACKGROUND-MATERIALS-METHODS: We initiated the studies reported in this paper to establish baseline growth data for tumors produced by several human brain tumor cell lines. Female athymic mice were injected with five established human glioblastoma cell lines subcutaneously. We optimized implantation conditions in SF-767 cells by evaluating tumor take and growth characteristics, and resulting growth data were compared to 2 other cell lines.
RESULTS-CONCLUSIONS: Three (SF-767, U-251 MG-NCI, and U-87 MG) of the 5 cell lines produced solid, ellipsoid tumors. For SF-767 cells, the best tumor growth parameters were achieved when 3.0 x 10(6) cells in 0.1 ml medium containing fetal calf serum were injected unilaterally. These conditions produced a high percentage of usable tumors (77.6%) that were detectable approximately 3 days after implantation and reached a size of 100 mm3 in 23 days. Comparison of several growth characteristics of the tumors produced by the 3 cell lines revealed that SF-767 tumors displayed the most uniform growth rates, the fastest doubling times, and the most uniform usable group of tumors (> 100 mm3). U-87 MG and U-251 MG-NCI had a similar histopathologic appearance while SF-767 had a different histology. Our results indicate that these 3 human glioblastoma cells produce flank tumors that exhibit decidedly different growth parameters. We are currently using all 3 of these human brain tumor xenograft models in other in vivo studies.
背景 - 材料 - 方法：我们开展了本文所报告的研究，以建立几种人类脑肿瘤细胞系产生的肿瘤的基线生长数据。将雌性无胸腺小鼠皮下注射五种已建立的人类胶质母细胞瘤细胞系。我们通过评估肿瘤接种和生长特征来优化SF - 767细胞的植入条件，并将所得生长数据与另外两种细胞系进行比较。
结果 - 结论：5种细胞系中的3种（SF - 767、U - 251 MG - NCI和U - 87 MG）产生了实体椭圆形肿瘤。对于SF - 767细胞，当在0.1 ml含胎牛血清的培养基中单侧注射3.0×10⁶个细胞时，可获得最佳肿瘤生长参数。这些条件产生了高比例的可用肿瘤（77.6%），植入后约3天可检测到，23天达到100 mm³大小。对这3种细胞系产生的肿瘤的几种生长特征进行比较发现，SF - 767肿瘤显示出最均匀的生长速率、最快的倍增时间以及最均匀的可用肿瘤组（>100 mm³）。U - 87 MG和U - 251 MG - NCI具有相似的组织病理学外观，而SF - 767具有不同的组织学特征。我们的结果表明，这3种人类胶质母细胞瘤细胞产生的胁腹肿瘤表现出明显不同的生长参数。我们目前正在其他体内研究中使用所有这3种人类脑肿瘤异种移植模型。
