Connell C J
Anat Rec. 1976 Aug;185(4):389-401. doi: 10.1002/ar.1091850402.
Scanning electron microscopy (SEM) is a potent tool that is especially valuable in interpreting the three-dimensional relationships of cells within tissues. This type of information is obtainable from thin sections in transmission electron microscopy (TEM) only by reconstructions of serial sections. The arrangement of the interstitial cells of the testis in relation to the capillaries and lymphatic channels, in particular, is easier to visualize in SEM than in TEM. Cytoplasmic constituents, as well as cell surface modifications, are demonstrable by this technique. The presence of droplets, presumably lipid droplets, both within and on the Leydig cells and the lymphatic endothelial cells, is quite evident. Other cytoplasmic structures are also apparent. For example, the possible functional significance of "openings" that are seen by SEM on the septa that surround lipid droplets is discussed relative to the appearance of the same area as seen in thin sections or in freeze-fracture replicas. SEM should become a very useful method for studying cytological and morphological alterations that occur in testicular tissue that is subjected to physical or chemical manipulation.
扫描电子显微镜（SEM）是一种强大的工具，在解释组织内细胞的三维关系方面具有特别重要的价值。这类信息只有通过连续切片重建才能从透射电子显微镜（TEM）的薄切片中获得。特别是，睾丸间质细胞相对于毛细血管和淋巴管的排列，在扫描电子显微镜下比在透射电子显微镜下更容易观察到。通过这种技术可以显示细胞质成分以及细胞表面修饰。在睾丸间质细胞和淋巴管内皮细胞内及表面存在的液滴，推测为脂滴，非常明显。其他细胞质结构也很明显。例如，相对于在薄切片或冷冻断裂复制品中看到的同一区域的外观，讨论了扫描电子显微镜在围绕脂滴的隔膜上看到的“开口”可能具有的功能意义。扫描电子显微镜应该成为研究受到物理或化学操作的睾丸组织中发生的细胞学和形态学改变的一种非常有用的方法。
