Johansson M L, Nobaek S, Berggren A, Nyman M, Björck I, Ahrné S, Jeppsson B, Molin G
Probi AB, Ideon Gamma 1, Lund, Sweden.
Int J Food Microbiol. 1998 Jun 30;42(1-2):29-38. doi: 10.1016/s0168-1605(98)00055-5.
In a controlled and randomised double-blind study, 26 healthy adult volunteers consumed, for 21 d, 400 ml of a rose-hip drink containing oats (0.7 g/100ml) fermented with Lactobacillus plantarum DSM 9843 (RHL; containing 5 x 10(7) cfu ml(-1)), and 22 volunteers in a second group the same amount of a pure rose-hip drink (RH). Significant increases in the total faecal concentration of carboxylic acids (P < 0.05 after 1 week and P < 0.01 after 3 weeks of intake), acetic acid (P < 0.01 after 3 weeks of intake) and propionic acid (P < 0.01 after 3 weeks of intake and P < 0.05 eight days after intake ceased) were recorded in the RHL group, indicating increased fermentation in the colon. In both groups a significant increase was obtained in the concentration of faecal lactic acid (P < 0.001 after 1 and 3 weeks of intake). No changes were seen in the concentration of faecal butyrate. The numbers of faecal bifidobacteria and lactobacilli increased significantly in both groups after 3 weeks of intake. Sulphite-reducing clostridia rapidly decreased in the group receiving the product with Lb. plantarum DSM 9843 after 1 week of intake, and then also in the pure rose-hip group after 3 weeks of intake. No changes were seen in the numbers of total anaerobes, gram-negative anaerobes or total aerobes during administration. Lb. plantarum DSM 9843 was recovered in faeces from all volunteers in the RHL group. Median amounts were 7.0 (5.0-8.8) log10 cfu g(-1) after one week of intake, and 6.7 (5.0-8.9) log10 cfu g(-1) after 3 weeks, respectively. The strain was still recovered from faeces of five volunteers 8 d after administration ceased (> 4.8 log10 cfu g(-1)). During the period of intake the volunteers in the RHL group experienced a significant increase in stool volume, a significant decrease in flatulence and slightly softer stools. Volunteers in the RH group experienced a slight but significant decrease in stool volume.
在一项对照随机双盲研究中，26名健康成年志愿者连续21天每天饮用400毫升含有燕麦（0.7克/100毫升）且经植物乳杆菌DSM 9843发酵的玫瑰果饮品（RHL，每毫升含5×10⁷ 菌落形成单位），另一组22名志愿者饮用等量的纯玫瑰果饮品（RH）。RHL组中，羧酸的粪便总浓度（摄入1周后P<0.05，摄入3周后P<0.01）、乙酸（摄入3周后P<0.01）和丙酸（摄入3周后P<0.01，停止摄入8天后P<0.05）显著增加，表明结肠发酵增强。两组中粪便乳酸浓度均显著增加（摄入1周和3周后P<0.001）。粪便丁酸浓度无变化。摄入3周后，两组中粪便双歧杆菌和乳酸杆菌数量均显著增加。摄入含植物乳杆菌DSM 9843产品的组中，摄入1周后亚硫酸盐还原梭菌迅速减少，3周后纯玫瑰果饮品组中也减少。给药期间，总厌氧菌、革兰氏阴性厌氧菌或需氧菌总数无变化。RHL组所有志愿者的粪便中均能检测到植物乳杆菌DSM 9843。摄入1周后中位数为7.0（5.0 - 8.8）log₁₀ 菌落形成单位/克，3周后为6.7（5.0 - 8.9）log₁₀ 菌落形成单位/克。停止给药8天后，仍有5名志愿者的粪便中能检测到该菌株（>4.8 log₁₀ 菌落形成单位/克）。在摄入期间，RHL组志愿者的粪便体积显著增加，肠胃胀气显著减少，粪便略变软。RH组志愿者的粪便体积略有但显著减少。
