白色念珠菌的KRE9基因是细胞壁β-1,6-葡聚糖合成所必需的，并且对在葡萄糖上生长至关重要。
The Candida albicans KRE9 gene is required for cell wall beta-1, 6-glucan synthesis and is essential for growth on glucose.
作者信息
Lussier M, Sdicu A M, Shahinian S, Bussey H
机构信息
Department of Biology, McGill University, 1205 Dr. Penfield Avenue, Montreal, Quebec, Canada, H3A 1B1.
出版信息
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9825-30. doi: 10.1073/pnas.95.17.9825.
Abstract
We have isolated CaKRE9, a gene from Candida albicans, that is a functional homologue of the Saccharomyces cerevisiae KRE9 gene involved in beta-1,6-glucan synthesis. Disruption of the CaKRE9 gene in C. albicans shows that CaKre9p is required for the synthesis or assembly of this fungal polymer. Homozygous null disruptants of CaKRE9 grow poorly on galactose and fail to form hyphae in serum, and, in growth medium containing glucose, the gene is essential. Thus, the CaKRE9 gene product is a potentially useful candidate as a target for fungal-specific drugs.
摘要
我们已经从白色念珠菌中分离出CaKRE9基因，它是酿酒酵母中参与β-1,6-葡聚糖合成的KRE9基因的功能同源物。白色念珠菌中CaKRE9基因的破坏表明CaKre9p对于这种真菌聚合物的合成或组装是必需的。CaKRE9的纯合无效突变体在半乳糖上生长不良，并且在血清中不能形成菌丝，而且在含有葡萄糖的生长培养基中，该基因是必需的。因此，CaKRE9基因产物作为真菌特异性药物的靶点是一个潜在的有用候选物。
相似文献
1
The Candida albicans KRE9 gene is required for cell wall beta-1, 6-glucan synthesis and is essential for growth on glucose.白色念珠菌的KRE9基因是细胞壁β-1,6-葡聚糖合成所必需的，并且对在葡萄糖上生长至关重要。
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9825-30. doi: 10.1073/pnas.95.17.9825.
2
Isolation of the Candida albicans homologs of Saccharomyces cerevisiae KRE6 and SKN1: expression and physiological function.酿酒酵母KRE6和SKN1的白色念珠菌同源物的分离：表达及生理功能
J Bacteriol. 1997 Apr;179(7):2363-72. doi: 10.1128/jb.179.7.2363-2372.1997.
3
The KNH1 gene of Saccharomyces cerevisiae is a functional homolog of KRE9.酿酒酵母的KNH1基因是KRE9的功能同源物。
Yeast. 1996 Jun 15;12(7):683-92. doi: 10.1002/(SICI)1097-0061(19960615)12:7%3C683::AID-YEA959%3E3.0.CO;2-8.
4
Isolation of Candida glabrata homologs of the Saccharomyces cerevisiae KRE9 and KNH1 genes and their involvement in cell wall beta-1,6-glucan synthesis.酿酒酵母KRE9和KNH1基因的光滑念珠菌同源物的分离及其在细胞壁β-1,6-葡聚糖合成中的作用。
J Bacteriol. 1998 Oct;180(19):5020-9. doi: 10.1128/JB.180.19.5020-5029.1998.
5
Isolation from Candida albicans of a functional homolog of the Saccharomyces cerevisiae KRE1 gene, which is involved in cell wall beta-glucan synthesis.从白色念珠菌中分离出酿酒酵母KRE1基因的功能同源物，该基因参与细胞壁β-葡聚糖的合成。
J Bacteriol. 1991 Nov;173(21):6859-64. doi: 10.1128/jb.173.21.6859-6864.1991.
6
Cloning of the RHO1 gene from Candida albicans and its regulation of beta-1,3-glucan synthesis.白色念珠菌RHO1基因的克隆及其对β-1,3-葡聚糖合成的调控。
J Bacteriol. 1997 Dec;179(24):7734-41. doi: 10.1128/jb.179.24.7734-7741.1997.
7
The yeast KRE9 gene encodes an O glycoprotein involved in cell surface beta-glucan assembly.酵母KRE9基因编码一种参与细胞表面β-葡聚糖组装的O-糖蛋白。
Mol Cell Biol. 1993 Oct;13(10):6346-56. doi: 10.1128/mcb.13.10.6346-6356.1993.
8
Cloning of the Candida albicans homolog of Saccharomyces cerevisiae GSC1/FKS1 and its involvement in beta-1,3-glucan synthesis.白色念珠菌酿酒酵母GSC1/FKS1同源物的克隆及其在β-1,3-葡聚糖合成中的作用。
J Bacteriol. 1997 Jul;179(13):4096-105. doi: 10.1128/jb.179.13.4096-4105.1997.
9
Characterization of the yeast (1-->6)-beta-glucan biosynthetic components, Kre6p and Skn1p, and genetic interactions between the PKC1 pathway and extracellular matrix assembly.酵母（1→6）-β-葡聚糖生物合成成分Kre6p和Skn1p的特性，以及PKC1途径与细胞外基质组装之间的遗传相互作用。
J Cell Biol. 1994 Oct;127(2):567-79. doi: 10.1083/jcb.127.2.567.
10
A mutational analysis of killer toxin resistance in Saccharomyces cerevisiae identifies new genes involved in cell wall (1-->6)-beta-glucan synthesis.酿酒酵母中杀伤毒素抗性的突变分析鉴定出参与细胞壁（1→6）-β-葡聚糖合成的新基因。
Genetics. 1993 Apr;133(4):837-49. doi: 10.1093/genetics/133.4.837.
引用本文的文献
1
Stress responsive glycosylphosphatidylinositol-anchored protein SsGSP1 contributes to virulence.应激反应性糖基磷脂酰肌醇锚定蛋白SsGSP1有助于毒力。
Virulence. 2025 Dec;16(1):2503434. doi: 10.1080/21505594.2025.2503434. Epub 2025 May 18.
2
Echinocandin Adaptation in Is Accompanied by Altered Chromatin Accessibility at Gene Promoters and by Cell Wall Remodeling.棘白菌素适应性伴随着基因启动子处染色质可及性的改变以及细胞壁重塑。
J Fungi (Basel). 2025 Feb 1;11(2):110. doi: 10.3390/jof11020110.
3
Fluconazole and Lipopeptide Surfactin Interplay During Plasma Membrane and Cell Wall Remodeling Increases Fungal Immune System Exposure.氟康唑与脂肽表面活性素在质膜和细胞壁重塑过程中的相互作用增加了真菌免疫系统暴露。
Pharmaceutics. 2020 Apr 1;12(4):314. doi: 10.3390/pharmaceutics12040314.
4
The cellular roles of Ccr4-NOT in model and pathogenic fungi-implications for fungal virulence.细胞中 Ccr4-NOT 在模式真菌和病原真菌中的作用——对真菌毒力的影响。
Front Genet. 2013 Dec 20;4:302. doi: 10.3389/fgene.2013.00302. eCollection 2013.
5
Effect of beta-1,6-glucan inhibitors on the invasion process of Candida albicans: potential mechanism of their in vivo efficacy.β-1,6-葡聚糖抑制剂对白色念珠菌侵袭过程的影响：其体内疗效的潜在机制
Antimicrob Agents Chemother. 2009 Sep;53(9):3963-71. doi: 10.1128/AAC.00435-09. Epub 2009 Jul 13.
6
Cell wall beta-(1,6)-glucan of Saccharomyces cerevisiae: structural characterization and in situ synthesis.酿酒酵母细胞壁β-（1,6）-葡聚糖：结构表征与原位合成
J Biol Chem. 2009 May 15;284(20):13401-13412. doi: 10.1074/jbc.M807667200. Epub 2009 Mar 11.
7
Discovery of a small-molecule inhibitor of {beta}-1,6-glucan synthesis.β-1,6-葡聚糖合成小分子抑制剂的发现。
Antimicrob Agents Chemother. 2009 Feb;53(2):670-7. doi: 10.1128/AAC.00844-08. Epub 2008 Nov 17.
8
Candida albicans cell wall proteins.白色念珠菌细胞壁蛋白。
Microbiol Mol Biol Rev. 2008 Sep;72(3):495-544. doi: 10.1128/MMBR.00032-07.
9
Deletion of the CaBIG1 gene reduces beta-1,6-glucan synthesis, filamentation, adhesion, and virulence in Candida albicans.CaBIG1基因的缺失会降低白色念珠菌中β-1,6-葡聚糖的合成、丝状化、黏附及毒力。
Infect Immun. 2006 Apr;74(4):2373-81. doi: 10.1128/IAI.74.4.2373-2381.2006.
10
KRE5 gene null mutant strains of Candida albicans are avirulent and have altered cell wall composition and hypha formation properties.白色念珠菌的KRE5基因缺失突变株无致病性，且其细胞壁组成和菌丝形成特性发生了改变。
Eukaryot Cell. 2004 Dec;3(6):1423-32. doi: 10.1128/EC.3.6.1423-1432.2004.
本文引用的文献
1
Resistance of clinically important yeasts to antifungal agents.临床上重要的酵母菌对抗真菌药物的耐药性。
Int J Antimicrob Agents. 1996 Feb;6(3):145-7. doi: 10.1016/0924-8579(95)00048-8.
2
Epidemiological shifts in opportunistic and nosocomial Candida infections: mycological aspects.机会性和医院获得性念珠菌感染的流行病学变化：真菌学方面。
Int J Antimicrob Agents. 1996 Feb;6(3):141-4. doi: 10.1016/0924-8579(95)00049-6.
3
The complete genome sequence of Escherichia coli K-12.大肠杆菌K-12的全基因组序列。
Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
4
Architecture of the yeast cell wall. Beta(1-->6)-glucan interconnects mannoprotein, beta(1-->)3-glucan, and chitin.酵母细胞壁的结构。β(1→6)-葡聚糖连接甘露糖蛋白、β(1→3)-葡聚糖和几丁质。
J Biol Chem. 1997 Jul 11;272(28):17762-75. doi: 10.1074/jbc.272.28.17762.
5
beta-Glucosylated proteins in the cell wall of the black yeast Exophiala (Wangiella) dermatitidis.黑色酵母菌皮炎外瓶霉（万吉外瓶霉）细胞壁中的β-葡萄糖基化蛋白。
Microbiology (Reading). 1997 May;143 ( Pt 5):1673-1680. doi: 10.1099/00221287-143-5-1673.
6
Isolation of the Candida albicans homologs of Saccharomyces cerevisiae KRE6 and SKN1: expression and physiological function.酿酒酵母KRE6和SKN1的白色念珠菌同源物的分离：表达及生理功能
J Bacteriol. 1997 Apr;179(7):2363-72. doi: 10.1128/jb.179.7.2363-2372.1997.
7
Susceptibilities of Candida albicans multidrug transporter mutants to various antifungal agents and other metabolic inhibitors.白色念珠菌多药转运蛋白突变体对各种抗真菌剂和其他代谢抑制剂的敏感性。
Antimicrob Agents Chemother. 1996 Oct;40(10):2300-5. doi: 10.1128/AAC.40.10.2300.
8
Life with 6000 genes.拥有6000个基因的生命。
Science. 1996 Oct 25;274(5287):546, 563-7. doi: 10.1126/science.274.5287.546.
9
The KNH1 gene of Saccharomyces cerevisiae is a functional homolog of KRE9.酿酒酵母的KNH1基因是KRE9的功能同源物。
Yeast. 1996 Jun 15;12(7):683-92. doi: 10.1002/(SICI)1097-0061(19960615)12:7%3C683::AID-YEA959%3E3.0.CO;2-8.
10
Nosocomial candidiasis: emerging species, reservoirs, and modes of transmission.医院念珠菌病：新出现的菌种、储存宿主及传播方式。
Clin Infect Dis. 1996 May;22 Suppl 2:S89-94. doi: 10.1093/clinids/22.supplement_2.s89.
Zotero 插件