Lussier M, Sdicu A M, Shahinian S, Bussey H
Department of Biology, McGill University, 1205 Dr. Penfield Avenue, Montreal, Quebec, Canada, H3A 1B1.
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9825-30. doi: 10.1073/pnas.95.17.9825.
We have isolated CaKRE9, a gene from Candida albicans, that is a functional homologue of the Saccharomyces cerevisiae KRE9 gene involved in beta-1,6-glucan synthesis. Disruption of the CaKRE9 gene in C. albicans shows that CaKre9p is required for the synthesis or assembly of this fungal polymer. Homozygous null disruptants of CaKRE9 grow poorly on galactose and fail to form hyphae in serum, and, in growth medium containing glucose, the gene is essential. Thus, the CaKRE9 gene product is a potentially useful candidate as a target for fungal-specific drugs.
我们已经从白色念珠菌中分离出CaKRE9基因，它是酿酒酵母中参与β-1,6-葡聚糖合成的KRE9基因的功能同源物。白色念珠菌中CaKRE9基因的破坏表明CaKre9p对于这种真菌聚合物的合成或组装是必需的。CaKRE9的纯合无效突变体在半乳糖上生长不良，并且在血清中不能形成菌丝，而且在含有葡萄糖的生长培养基中，该基因是必需的。因此，CaKRE9基因产物作为真菌特异性药物的靶点是一个潜在的有用候选物。
