Parish M E
Citrus Research and Education Center, University of Florida, Lake Alfred 33850, USA.
J Food Prot. 1998 Mar;61(3):280-4. doi: 10.4315/0362-028x-61.3.280.
A salmonellosis outbreak occurred during the summer of 1995 among individuals who consumed nonpasteurized orange juice from a Florida citrus-processing facility. Clinical isolates were identified by the Centers for Disease Control as Salmonella serovars Hartford, Gaminara, and Rubislaw. At the processing facility, 70 samples (equipment swabs, fruit surface swabs, juice, and miscellaneous environmental samples) were collected before, during, and after processing runs on two different dates. Bottled juice samples from eight previous extraction dates were also collected. Total plate counts, fecal coliforms, and Escherichia coli were enumerated from each sample. Analysis for Salmonella cells were conducted on all juice samples, fruit surface swabs, environmental samples, and selected equipment swabs using direct enrichment and pre-enrichment techniques. Salmonella serovars Hartford, Rubislaw, Saintpaul, and Newport were detected from either juice, unwashed fruit surfaces, or amphibians (Hyla cinerea and Bufo terrestris) captured outside the processing building. Salmonella cells in juice were associated with population levels of fecal coliforms and E. coli above the upper most probable number (MPN) limits of detection (> 110/ml).
1995年夏天，食用了佛罗里达州一家柑橘加工设施生产的未杀菌橙汁的人群中爆发了沙门氏菌病疫情。疾病控制中心将临床分离株鉴定为沙门氏菌血清型哈特福德、加米纳拉和鲁比斯拉夫。在该加工设施，于两个不同日期的加工运行前、运行期间和运行后采集了70份样本（设备拭子、水果表面拭子、果汁和其他环境样本）。还收集了此前八个提取日期的瓶装果汁样本。对每个样本进行了总平板计数、粪大肠菌群和大肠杆菌的计数。使用直接富集和预富集技术，对所有果汁样本、水果表面拭子、环境样本和选定的设备拭子进行了沙门氏菌细胞分析。从果汁、未清洗的水果表面或在加工车间外捕获的两栖动物（灰树蛙和北美蟾蜍）中检测到了沙门氏菌血清型哈特福德、鲁比斯拉夫、圣保罗和纽波特。果汁中的沙门氏菌细胞与粪大肠菌群和大肠杆菌的数量水平相关，这些数量高于检测的最高可能数（MPN）限值（>110/ml）。
