Nawrot B, Sprinzl M
Laboratorium für Biochemie, Universität Bayreuth, Germany.
Nucleosides Nucleotides. 1998 Apr;17(4):815-29. doi: 10.1080/07328319808004677.
Reaction of isatoic anhydride with adenosine, adenosine 5'-phosphate, oligoribonucleotides or with the E. coli tRNAVal led to attachment of an anthraniloyl residue at 2'- or 3'-OH groups of 3'-terminal ribose residue. No protection of the 5'-hydroxyl group or internal 2'-hydroxyl groups is required for this specific reaction. Anthraniloyl-tRNA which is an analogue of aminoacyl-tRNA forms a ternary complex with EF-Tu*GTP. The anthraniloyl-residue is used as a fluorescent reporter group to monitor interactions with proteins.
异吲哚酮酸酐与腺苷、5'-磷酸腺苷、寡核糖核苷酸或大肠杆菌缬氨酸转运核糖核酸（tRNAVal）反应，导致邻氨基苯甲酰残基连接到3'-末端核糖残基的2'-或3'-羟基上。该特异性反应不需要对5'-羟基或内部2'-羟基进行保护。作为氨酰转运核糖核酸类似物的邻氨基苯甲酰转运核糖核酸（Anthraniloyl-tRNA）与延伸因子Tu·鸟苷三磷酸（EF-Tu*GTP）形成三元复合物。邻氨基苯甲酰残基用作荧光报告基团以监测与蛋白质的相互作用。
