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野生型核糖体对多种芳纶和1,3 - 二羰基肽的翻译

Translation of Diverse Aramid- and 1,3-Dicarbonyl-peptides by Wild Type Ribosomes .

作者信息

Ad Omer, Hoffman Kyle S, Cairns Andrew G, Featherston Aaron L, Miller Scott J, Söll Dieter, Schepartz Alanna

机构信息

Department of Chemistry and Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, United States.

出版信息

ACS Cent Sci. 2019 Jul 24;5(7):1289-1294. doi: 10.1021/acscentsci.9b00460. Epub 2019 Jun 26.

DOI:10.1021/acscentsci.9b00460
PMID:31403077
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6661870/
Abstract

Here, we report that wild type ribosomes accept and elongate precharged initiator tRNAs acylated with multiple benzoic acids, including aramid precursors, as well as malonyl (1,3-dicarbonyl) substrates to generate a diverse set of aramid-peptide and polyketide-peptide hybrid molecules. This work expands the scope of ribozyme- and ribosome-catalyzed chemical transformations, provides a starting point for translation engineering efforts, and offers an alternative strategy for the biosynthesis of polyketide-peptide natural products.

摘要

在此，我们报道野生型核糖体能够接受并延长用多种苯甲酸（包括芳酰胺前体）以及丙二酰（1,3 - 二羰基）底物酰化的预充电起始tRNA，以生成多种芳酰胺 - 肽和聚酮 - 肽杂合分子。这项工作扩展了核酶和核糖体催化的化学转化的范围，为翻译工程研究提供了一个起点，并为聚酮 - 肽天然产物的生物合成提供了一种替代策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58f6/6661870/396e514a06db/oc-2019-00460j_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58f6/6661870/f208e4633672/oc-2019-00460j_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58f6/6661870/db6fdbc73c4b/oc-2019-00460j_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58f6/6661870/87e258d4534e/oc-2019-00460j_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58f6/6661870/3e96a6181eb1/oc-2019-00460j_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58f6/6661870/396e514a06db/oc-2019-00460j_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58f6/6661870/f208e4633672/oc-2019-00460j_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58f6/6661870/db6fdbc73c4b/oc-2019-00460j_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58f6/6661870/87e258d4534e/oc-2019-00460j_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58f6/6661870/3e96a6181eb1/oc-2019-00460j_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58f6/6661870/396e514a06db/oc-2019-00460j_0005.jpg

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