Koizumi M, Ozawa Y, Yagi R, Nishigaki T, Kaneko M, Oka S, Kimura S, Iwamoto A, Komatsu Y, Ohtsuka E
Exploratory Chemistry Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
Nucleosides Nucleotides. 1998 Jan-Mar;17(1-3):207-18. doi: 10.1080/07328319808005170.
Three ribozymes, a hairpin ribozyme (HR112) and two hammerhead ribozymes (RZ115 and RZ119) containing a 5'C(UUCG)G3' loop were designed to cleave the U5 region in the long terminal repeat (LTR) of HIV-1 RNA. The t1/2 values of chemically synthesized substrates mediated by three ribozymes were measured. The transformed CEM cells possessing these three ribozyme-encoding genes were challenged with a HIV-1IIIB strain, and two of these three ribozymes, HR112 and RZ119, were shown to possess strong anti-HIV-1 activity.
