合成核酶对HIV-1高度保守发夹环区域的切割
Cleavage of the highly conserved hairpin-loop region of HIV-1 by synthetic ribozymes.
作者信息
Shimayama T, Nishikawa S, Taira K
机构信息
National Institute of Bioscience and Human Technology, Agency of Industrial Science & Technology, MITI, Tsukuba Science City, Japan.
出版信息
Nucleic Acids Symp Ser. 1993(29):177-8.
Hammerhead ribozymes can be considered as molecular scissors for suppressing AIDS virus. It is attractive to target single-stranded region of HIV-1 RNA because single-stranded RNA interactions or "kissing" induce hybridization. We, therefore, chose a highly conserved hairpin-loop structure, that is located in the 5' long terminal repeat region and just downstream of the TAR regulatory sequence. As a model system, we synthesized the corresponding hairpin-loop structure (21-mer RNA) and several types of ribozyme that are different in sizes in the hybridizing arms. These ribozymes successfully cleaved the 21-mer hairpin-structured substrate after CUU sequence but with different efficiencies.
锤头状核酶可被视为抑制艾滋病病毒的分子剪刀。靶向HIV-1 RNA的单链区域很有吸引力，因为单链RNA相互作用或“亲吻”会诱导杂交。因此，我们选择了一个高度保守的发夹环结构，它位于5'长末端重复区域且恰好在TAR调控序列的下游。作为一个模型系统，我们合成了相应的发夹环结构（21聚体RNA）以及几种在杂交臂大小上不同的核酶类型。这些核酶成功地在CUU序列后切割了21聚体发夹结构的底物，但效率不同。
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