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5-硝基吲哚-2'-脱氧核糖-5'-三磷酸用于寡核苷酸的标记和检测。

Use of 5-nitroindole-2'-deoxyribose-5'-triphosphate for labelling and detection of oligonucleotides.

作者信息

Smith C L, Simmonds A C, Hamilton A L, Martin D L, Lashford A G, Loakes D, Hill F, Brown D M

机构信息

Amersham International plc, Amersham Laboratories, Buckinghamshire, UK.

出版信息

Nucleosides Nucleotides. 1998 Jan-Mar;17(1-3):555-64. doi: 10.1080/07328319808005198.

DOI:10.1080/07328319808005198
PMID:9708362
Abstract

The 5'-triphosphate of 5-nitroindole-2'-deoxyriboside has been shown to be a good substrate for terminal deoxynucleotidyl transferase (TdT). An antibody has been prepared for the detection of 5-nitroindole and has been used for the detection of 5-nitroindole tailed DNA both in single-stranded form and after hybridisation to a template. This is therefore a new method for the detection of nucleic acid probes.

摘要

5-硝基吲哚-2'-脱氧核糖核苷的5'-三磷酸已被证明是末端脱氧核苷酸转移酶（TdT）的良好底物。已制备了一种用于检测5-硝基吲哚的抗体，并已用于检测单链形式以及与模板杂交后的5-硝基吲哚尾DNA。因此，这是一种检测核酸探针的新方法。

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