Hess W R, Fingerhut C, Schön A
Institut für Biologie, Humboldt-Universität, Berlin, Germany.
FEBS Lett. 1998 Jul 17;431(2):138-42. doi: 10.1016/s0014-5793(98)00729-7.
The molecular organisation of the Prochlorococcus marinus rnpB gene and the catalytic activity of the encoded RNA were characterised. Kinetic parameters for several pre-tRNA substrates were comparable to those from other eubacterial RNase P RNAs, although unusually high cation concentrations were required. The CCA-end of pre-tRNAs is essential for efficient turnover despite the lack of the canonical binding motif in P. marinus RNase P RNA. A trnR gene is located only 38 nt upstream the rnpB 5' end on the complementary strand. This arrangement resembles those in the plastids of Cyanophora and Porphyra but not in any other bacterium.
对海洋原绿球藻rnpB基因的分子组织以及编码RNA的催化活性进行了表征。几种前体tRNA底物的动力学参数与其他真细菌核糖核酸酶P RNA的参数相当，尽管需要异常高的阳离子浓度。尽管海洋原绿球藻核糖核酸酶P RNA中缺乏典型的结合基序，但前体tRNA的CCA末端对于有效周转至关重要。一个trnR基因位于互补链上rnpB 5'末端上游仅38个核苷酸处。这种排列类似于蓝氏藻和紫菜质体中的排列，但在任何其他细菌中都不存在。
