Bauer L S, Miller D L, Maddox J V, McManus M L
Department of Entomology, North Central Forest Experiment Station, East Lansing, Michigan, 48823, USA.
J Invertebr Pathol. 1998 Sep;72(2):147-53. doi: 10.1006/jipa.1998.4773.
Simultaneous and sequential per os inoculations of gypsy moth larvae with the Lymantria dispar nuclear polyhedrosis virus (LdNPV) and a Nosema sp. from Portugal demonstrated that the interaction of two pathogens during coinfection was variable, ranging from synergistic to antagonistic. Susceptibility of gypsy moth larvae to viral infection was unaffected by simultaneous and subsequent microsporidian infection. This resulted from the comparatively slow pathogenesis of the microsporidium when compared to the virus. Viral infectivity, however, increased 10-fold when larvae were preinfected with Nosema sp. per os, or through transovarial infection. Time to death decreased for larvae infected with both pathogens compared to larvae infected with the virus alone. Polyhedron production was significantly reduced by microsporidian infection preceding viral infection. In this infection sequence, larvae died at an earlier stage and were less than half the mass of cadavers infected with virus alone. The biological significance of these results on gypsy moth population dynamics and the implication for use of this Nosema sp. from Portugal in gypsy moth biological control are discussed in the context of viral epizootiology.
用舞毒蛾核型多角体病毒（LdNPV）和一种来自葡萄牙的微孢子虫对舞毒蛾幼虫进行同时经口接种和序贯经口接种，结果表明两种病原体在共感染期间的相互作用是可变的，范围从协同到拮抗。舞毒蛾幼虫对病毒感染的易感性不受同时和随后微孢子虫感染的影响。这是由于与病毒相比，微孢子虫的致病过程相对较慢。然而，当幼虫经口或通过经卵巢感染预先感染微孢子虫时，病毒感染力增加了10倍。与仅感染病毒的幼虫相比，同时感染两种病原体的幼虫死亡时间缩短。在病毒感染之前的微孢子虫感染显著降低了多角体的产生。在这种感染顺序中，幼虫在早期死亡，其质量不到仅感染病毒的尸体质量的一半。在病毒流行病学的背景下，讨论了这些结果对舞毒蛾种群动态的生物学意义以及这种来自葡萄牙的微孢子虫在舞毒蛾生物防治中的应用前景。
