Malakar R, Elkinton JS, Hajek AE, Burand JP
Department of Entomology, University of Massachusetts, Amherst, Massachusetts, 01003, USA.
J Invertebr Pathol. 1999 Jan;73(1):91-100. doi: 10.1006/jipa.1998.4806.
The interaction of two gypsy moth (Lymantria dispar) pathogens, a nucleopolyhedrosis virus (LdNPV) and a fungus (Entomophaga maimaiga), were studied by assessing mortality among dually inoculated hosts. When fourth and fifth instar gypsy moths were inoculated with a range of doses of LdNPV and a fixed dose of E. maimaiga on the same day, the majority of larvae died from E. maimaiga infections regardless of the dose of LdNPV. When the larvae were inoculated with E. maimaiga 10 days after LdNPV, there was an apparent increase in mortality of hosts induced by LdNPV. Among the fourth instars, the mortality due to LdNPV in the presence of E. maimaiga was significantly higher when insects were reared at 25 than at 20 degreesC. At 25 degreesC, the LD50 of LdNPV for fifth instars was twofold greater than that for fourth instars. For those larvae that died from LdNPV, the median survival time (ST50) of dually inoculated fourth and fifth instars was ca. 1 day shorter than those inoculated with LdNPV alone. The number of LdNPV occlusion bodies produced in the cadavers from the dually inoculated larvae was lower than those inoculated with LdNPV alone. Copyright 1999 Academic Press.
通过评估双重接种宿主的死亡率，对两种舞毒蛾（Lymantria dispar）病原体——一种核型多角体病毒（LdNPV）和一种真菌（舞毒蛾噬虫霉Entomophaga maimaiga）之间的相互作用进行了研究。当在同一天用一系列剂量的LdNPV和固定剂量的舞毒蛾噬虫霉对四龄和五龄舞毒蛾进行接种时，无论LdNPV的剂量如何，大多数幼虫死于舞毒蛾噬虫霉感染。当幼虫在接种LdNPV 10天后接种舞毒蛾噬虫霉时，由LdNPV诱导的宿主死亡率明显增加。在四龄幼虫中，当在25℃而非20℃饲养昆虫时，在存在舞毒蛾噬虫霉的情况下，由LdNPV导致的死亡率显著更高。在25℃时，LdNPV对五龄幼虫的半数致死剂量（LD50）比对四龄幼虫的LD50大两倍。对于那些死于LdNPV的幼虫，双重接种的四龄和五龄幼虫的中位存活时间（ST50）比单独接种LdNPV的幼虫约短1天。双重接种幼虫尸体中产生的LdNPV包涵体数量低于单独接种LdNPV的幼虫。版权所有1999年学术出版社。
