Hsieh Y H, Sheu S C, Bridgman R C
Department of Nutrition and Food Science, Auburn University, Alabama 36849, USA.
J Food Prot. 1998 Apr;61(4):476-81. doi: 10.4315/0362-028x-61.4.476.
Detection of species adulteration in ground meat products is important for consumer protection and food-labeling law enforcement. This study was conducted to develop monoclonal antibodies (MAbs) that can be used in an enzyme-linked immunosorbent assay (ELISA) for rapid detection of any cooked mammalian meats in cooked poultry products. Soluble muscle proteins extracted from cooked pork (heated at 100 degrees C for 15 min) were used as the antigen to immunized mice for developing the MAb. One that was developed, MAb 2F8 (IgG2b class), strongly reacted with cooked meat of five mammalian species (beef cattle, hogs, sheep, horse, and deer) but did not react with any cooked poultry (chicken, turkey, and duck) or raw meats. At least 0.5% by weight of pork, beef, lamb, and horse meats in a chicken-based mixture could not detect using the indirect ELISA with MAb 2F8. The MAb 2F8 is useful in a single initial screening test to detect the presence of five nonpoultry meat adulterants in cooked poultry products.
检测碎肉产品中的物种掺假对于保护消费者和执行食品标签法很重要。本研究旨在开发可用于酶联免疫吸附测定（ELISA）的单克隆抗体（MAb），以快速检测熟禽产品中是否掺有任何熟哺乳动物肉。从熟猪肉（在100℃加热15分钟）中提取的可溶性肌肉蛋白用作抗原免疫小鼠以制备单克隆抗体。所开发的一种单克隆抗体2F8（IgG2b类）与五种哺乳动物的熟肉（肉牛、猪、羊、马和鹿）强烈反应，但与任何熟禽肉（鸡肉、火鸡和鸭肉）或生肉均无反应。使用基于单克隆抗体2F8的间接ELISA无法检测出鸡肉混合物中至少0.5%（重量）的猪肉、牛肉、羊肉和马肉。单克隆抗体2F8可用于单次初步筛选试验，以检测熟禽产品中是否存在五种非禽肉掺假物。
