Thienes Cortlandt P, Masiri Jongkit, Benoit Lora A, Barrios-Lopez Brianda, Samuel Santosh A, Krebs Richard A, Cox David P, Dobritsa Anatoly P, Nadala Cesar, Samadpour Mansour
Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155.
IEH Laboratories and Consulting Group, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155.
J AOAC Int. 2019 May 1;102(3):898-902. doi: 10.5740/jaoacint.18-0193. Epub 2018 Sep 18.
Concerns about the contamination of meat products with undeclared meats, and new regulations for the declaration of meat adulterants have established the need for a rapid test to detect beef adulteration to 0.1% sensitivity. To address this need, Microbiologique, Inc. has developed an ELISA that can quantify the presence of beef down to 0.1% (w/w) in cooked pork, horse, chicken, goat, and sheep meat. The beef-authentication ELISA has an analytical sensitivity of 0.00022 and 0.00012% (w/v) for cooked and autoclaved beef, respectively, and an analytical range of quantitation of 0.025 to 2% (w/v), in the absence of other meats. Moreover, the assay is specific for cooked beef and does not cross react with common food matrixes. The assay is rapid, can be completed in 70 min, and can detect a 0.1% level of meat adulteration. The assay is an improvement over a previous U.S. Department of Agriculture's tested assay, which is sensitive to 1% adulteration and takes 2.5-3 h to complete. The Microbiologique Cooked Beef ELISA can quantitate cooked beef in the presence of pork, horse, chicken, goat, and sheep meat to 0.1% (w/w) and is not affected by common food matrixes.
对肉类产品被未申报肉类污染的担忧，以及肉类掺假物申报的新规定，使得有必要开发一种快速检测方法，以检测牛肉掺假，灵敏度达到0.1%。为满足这一需求，微生物学公司开发了一种酶联免疫吸附测定法（ELISA），该方法能够在熟猪肉、马肉、鸡肉、羊肉和山羊肉中定量检测低至0.1%（重量/重量）的牛肉。牛肉鉴定ELISA对熟牛肉和高压灭菌牛肉的分析灵敏度分别为0.00022%和0.00012%（重量/体积），在不存在其他肉类的情况下，定量分析范围为0.025%至2%（重量/体积）。此外，该检测方法对熟牛肉具有特异性，不会与常见的食物基质发生交叉反应。该检测方法速度快，70分钟即可完成，能够检测出0.1%水平的肉类掺假。该检测方法相较于美国农业部之前测试的方法有所改进，之前的方法对1%的掺假敏感，需要2.5至3小时才能完成。微生物学熟牛肉ELISA能够在存在猪肉、马肉、鸡肉、羊肉和山羊肉的情况下，将熟牛肉定量检测至0.1%（重量/重量），且不受常见食物基质的影响。
