Zagulski T, Lipiński P, Zagulska A, Jarzabek Z
Polish Academy of Sciences, Department of Molecular Biology, Mrokow, Poland.
Int J Exp Pathol. 1998 Apr;79(2):117-23. doi: 10.1046/j.1365-2613.1998.00058.x.
A single dose of bovine lactoferrin (BLF) was given intravenously (i.v.) to CFW mice 24 hours (h) prior to the i.v. injection of the E. coli lethal dose (LD100). BLF strongly accelerated the clearance rate of E. coli from the blood as well as its killing rate in the liver, lungs, spleen and kidney. The highest clearing and killing rate was found 5 h after E. coli LD100 injection. The most intensive killing in the organs examined was found in the lungs and kidney. Analysis of organs of i.v. BLF-stimulated mice which survived up to day 30 after the infection by E. coli showed that not all animals were definitely pathogen-free. It was concluded that the defense system generated by BLF in mice in vivo is primarily a bacteria-killing one. The participation and cooperation of reticulo-endothelial (RE)-macrophages and granulocytes in the phagocytosis and killing of E. coli may thus be related to the protective activity of LF.
在静脉注射大肠杆菌致死剂量（LD100）前24小时，给CFW小鼠静脉注射单剂量牛乳铁蛋白（BLF）。BLF显著加快了大肠杆菌从血液中的清除率以及其在肝脏、肺、脾脏和肾脏中的杀灭率。在注射大肠杆菌LD100后5小时，清除率和杀灭率最高。在所检查的器官中，肺和肾脏中的杀灭作用最为强烈。对在感染大肠杆菌后存活至第30天的静脉注射BLF刺激小鼠的器官分析表明，并非所有动物都绝对无病原体。得出的结论是，BLF在小鼠体内产生的防御系统主要是一种细菌杀灭系统。因此，网状内皮（RE）巨噬细胞和粒细胞在吞噬和杀灭大肠杆菌中的参与和协作可能与乳铁蛋白的保护活性有关。
