Programa Regional para el Doctorado en Biotecnología, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Sinaloa, Culiacán Sinaloa, México.
Biochem Cell Biol. 2012 Jun;90(3):405-11. doi: 10.1139/o11-089. Epub 2012 Feb 14.
Mice orally infected with enterohaemorrhagic Escherichia coli (EHEC) O157:H7 were used to evaluate the activity of bovine lactoferrin (bLF) and the synthetic peptide LFchimera. Groups of BALB/c mice inoculated intragastrically with EHEC O157:H7 showed chronic intestinal infection with the pathogen that persisted over 6 days and resulted in a high mortality rate (90%). LFchimera and kanamycin significantly decreased (40%) this mortality rate (P = 0.028). On the other hand, although mice administered with bLF showed an important reduction in mortality (50%), this was not statistically significant (P = 0.070). In infected and untreated mice, severe tubular necrosis, glomerular lesions, and moderate intratubular hyaline casts were found in the kidney. However, in the bLF and LFchimera groups we found a reduction in the damage and a substantial decrease in the bacterial concentration excreted in feces 48 h after infection. Furthermore, sepsis caused by EHEC was reduced by the treatments, evidenced by the fact that bacteria were not detected in the kidney or liver 72 h after infection. The results suggest the bLF and LFchimera could have potential as therapeutics in EHEC infections.
经口感染产肠出血性大肠杆菌（EHEC）O157：H7 的小鼠用于评估牛乳铁蛋白（bLF）和合成肽 LFchimera 的活性。通过胃内接种 EHEC O157：H7 感染 BALB/c 小鼠的组显示出与病原体的慢性肠道感染，持续超过 6 天，并导致高死亡率（90%）。LFchimera 和卡那霉素显著降低（40%）了这种死亡率（P = 0.028）。另一方面，尽管给予 bLF 的小鼠死亡率显著降低（50%），但这在统计学上并不显著（P = 0.070）。在感染和未治疗的小鼠中，肾脏中发现严重的肾小管坏死、肾小球病变和中度管内透明管型。然而，在 bLF 和 LFchimera 组中，我们发现损伤减少，感染后 48 小时粪便中排出的细菌浓度显著降低。此外，EHEC 引起的败血症通过治疗得到了减轻，这一事实表明感染后 72 小时，肾脏或肝脏中未检测到细菌。结果表明，bLF 和 LFchimera 可能具有治疗 EHEC 感染的潜力。
