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储存时间和温度以及(不同日期的)重复试验之间的差异对芽孢盘和试纸性能的影响。

Effect of storage time and temperature and the variation among replicate tests (on different days) on the performance of spore disks and strips.

作者信息

Smith G M, Pflug I J, Chapman P A

出版信息

Appl Environ Microbiol. 1976 Aug;32(2):257-63. doi: 10.1128/aem.32.2.257-263.1976.

Abstract

Laboratory-prepared spore disks were stored for 96 weeks at 22 degrees C with 50% relative humidity (RH) and at 4 degrees C with less than 1% RH. At the same time commercial spore strips were stored for 64 weeks at 22 degrees C with 50% RH. The spore count per unit and the heat resistance were measured at the beginning of the experiment and after 16, 32, 48, 64, 80, and 96 weeks of storage. The laboratory-prepared spore disks stored at 4 degrees C with less than 1% RH showed less change in numbers of spores per disks and decrease in the survival time than did the disks stored at 22 degrees C with 50% RH. Both the laboratory-prepared spore disks and the commercial spore strips stored at 22 degrees C with 50% RH decreased in survival times with increased storage time. The relative change in the survival times with storage was less for the commercial spore strips than for the laboratory-prepared spore disks.

摘要

实验室制备的孢子盘在22摄氏度、相对湿度50%(RH)的条件下储存96周,以及在4摄氏度、相对湿度低于1%的条件下储存。同时,商用孢子条在22摄氏度、相对湿度50%的条件下储存64周。在实验开始时以及储存16、32、48、64、80和96周后,测量每单位的孢子计数和耐热性。与在22摄氏度、相对湿度50%的条件下储存的孢子盘相比,在4摄氏度、相对湿度低于1%的条件下储存的实验室制备的孢子盘每盘孢子数量变化较小,存活时间缩短。在22摄氏度、相对湿度50%的条件下储存的实验室制备的孢子盘和商用孢子条,其存活时间均随储存时间延长而缩短。与实验室制备的孢子盘相比,商用孢子条存活时间随储存的相对变化较小。

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