Kanda T, Ochi M, Ikuta Y
Department of Orthopaedic Surgery, Hiroshima University School of Medicine, Japan.
Arch Orthop Trauma Surg. 1998;117(6-7):307-11. doi: 10.1007/s004020050256.
Changes in the permeability of horseradish peroxidase (HRP) and the histology of the rabbit anterior cruciate ligament (ACL) caused by knee immobilization were studied. The knees of adult rabbits were immobilized for 2-8 weeks by a long leg cast with the knee flexed at 120 degrees, and some joints were allowed to be remobilized for 4 weeks after 6 or 8 weeks' immobilization. After 1 ml of HRP was injected into the knee joint and allowed to permeate into the ACL, the degree of permeation at the mid-portion of the ACL between femur and tibia was visualized with diaminobenzidine tetrahydrochloride. The ACL was examined histologically by light and transmission electron microscopy. Although the HRP permeated into almost the entire area of the transverse section of the non-immobilized normal ACL, as the period of immobilization increased, the extent of permeation gradually decreased. The decrease was significant from week 4 to week 6 and from week 6 to week 8. Degenerative change with necrosis of cells at the central area of the ACL was noted in the 6-week immobilization group, and the 8-week period of immobilization increased the extent of this degeneration. Although the permeation recovered in both the 6-week and 8-week immobilization groups 4 weeks after remobilization, there were fewer cells with a normal morphology in the central area in the 8-week immobilization group than in the control. These results suggest that long-term immobilization adversely affects the ACL.
研究了膝关节固定对辣根过氧化物酶（HRP）通透性及兔前交叉韧带（ACL）组织学的影响。成年兔膝关节用长腿石膏固定于120度屈曲位2 - 8周，部分关节在固定6周或8周后再活动4周。向膝关节内注射1ml HRP并使其渗透至ACL后，用二氨基联苯胺四盐酸盐观察股骨和胫骨之间ACL中部的渗透程度。通过光镜和透射电镜对ACL进行组织学检查。尽管HRP可渗透至未固定的正常ACL横切面的几乎整个区域，但随着固定时间的增加，渗透范围逐渐减小。从第4周至第6周以及从第6周至第8周，这种减小具有显著性。在6周固定组中，观察到ACL中央区域细胞坏死的退行性改变，8周固定期增加了这种退变的程度。尽管在再活动4周后，6周和8周固定组的渗透均恢复，但8周固定组中央区域形态正常的细胞比对照组少。这些结果表明长期固定对ACL有不利影响。
