Ochi M, Kanda T, Sumen Y, Ikuta Y
Department of Orthopaedic Surgery, Hiroshima University School of Medicine, Japan.
Clin Orthop Relat Res. 1997 Jan(334):305-15.
The knees of mature rabbits were immobilized for 2 to 8 weeks by a long leg cast to examine adverse effects on the meniscus. Some joints were remobilized for 1 to 4 weeks after an immobilization period of either 6 or 8 weeks. Horseradish peroxidase was injected into knee joints, and the degree of permeation into the meniscus was visualized with diaminobenzidine tetrahydrochloride. Menisci were examined histologically by light and transmission electron microscopic study. Injected horseradish peroxidase permeated all areas of the meniscus in nonimmobilized joints. However, permeability of horseradish peroxidase gradually decreased with increasing time of immobilization and was restricted to the superficial layer of the meniscus in the 8-week immobilization group. Degenerative changes in the deep layers of the meniscus were noted in the 6-week immobilization group: 8 weeks of immobilization induced additional degenerative changes. Although permeability of horseradish peroxidase gradually recovered with remobilization, the degenerative changes remained in the deep meniscal layer in the 8-week immobilization group, even after 4 weeks of remobilization. These results clearly show that long-term immobilization of the knee joint causes adverse changes in the meniscus.
通过长腿石膏固定成年兔的膝关节2至8周，以检查对半月板的不良影响。在6周或8周的固定期后，一些关节再活动1至4周。将辣根过氧化物酶注入膝关节，并用二氨基联苯胺四盐酸盐观察其向半月板的渗透程度。通过光镜和透射电镜研究对半月板进行组织学检查。在未固定的关节中，注入的辣根过氧化物酶渗透到半月板的所有区域。然而，随着固定时间的增加，辣根过氧化物酶的通透性逐渐降低，在8周固定组中仅限于半月板的表层。在6周固定组中观察到半月板深层的退行性变化；8周固定导致额外的退行性变化。尽管随着再活动辣根过氧化物酶的通透性逐渐恢复，但即使在再活动4周后，8周固定组半月板深层的退行性变化仍然存在。这些结果清楚地表明，膝关节的长期固定会导致半月板发生不良变化。
