Kumasaka T, Nishi N, Yaoi Y, Kido Y, Suzuki A, Saito M, Okayasu I, Hatakeyama S, Sawano S, Kokubu T
Nihon Naibunpi Gakkai Zasshi. 1978 Jun 20;54(6):779-86. doi: 10.1507/endocrine1927.54.6_779.
Since Arimura et al (1975) reported the radioimmunoassay for somatostatin (GIF), the concentration of GIF in various organ and brain regions were determined by radio-immunoassay. Dubois et al (1975) reported that immunohistochemically somatostatin was located in the discrete cells of the pancreas as well as the hypothalamus, and from this result, they presented the concept of local hormone instead of systemic hormone which was up to that time accepted in endocrinology. In this study, we developed the high specific anti-GIF serum using rabbits, and with the micro immunodiffusion method, we demonstrated that the precipitin band formed a circular fusion between the GIF and anti-GIF serum. This pattern of reaction was also seen in decidual immunodiffusion. In addition, we developed the radioimmunoassay for GIF using this anti-serum and measured immunoreactive GIF-like substances in villi and decidua of early pregnancy. The concentration of GIF-like substances with 2 N acetic acid extracted of villi and decidua were 0 to 30 pg/0.1 g dry weight. At the same time, we demonstrated the presence of GIF-like substance-containing cells in the villi and decidua by indirect immunofluorescent method. The intensity of immunofluorescence was in cytotrophoblast rather than syncytiotrophoblast, and decidual stromal cell also reacted to the immunofluorescence.
自从有村等人（1975年）报道了生长抑素（GIF）的放射免疫测定法以来，各种器官和脑区中GIF的浓度已通过放射免疫测定法得以确定。杜波依斯等人（1975年）报道，免疫组织化学研究表明生长抑素位于胰腺以及下丘脑的离散细胞中，基于这一结果，他们提出了局部激素的概念，以取代当时内分泌学中所认可的全身激素概念。在本研究中，我们用兔子制备了高特异性抗GIF血清，并采用微量免疫扩散法证明，沉淀带在GIF和抗GIF血清之间形成了环形融合。这种反应模式在蜕膜免疫扩散中也可见到。此外，我们利用这种抗血清开发了GIF的放射免疫测定法，并测定了早孕绒毛和蜕膜中具有免疫反应性的GIF样物质。用2N乙酸从绒毛和蜕膜中提取的GIF样物质的浓度为0至30 pg/0.1 g干重。同时，我们通过间接免疫荧光法证明了绒毛和蜕膜中存在含GIF样物质的细胞。免疫荧光强度在细胞滋养层而非合体滋养层中较强，蜕膜基质细胞也对免疫荧光有反应。
