Imakita T, Kumasaka T, Suzuki A, Saito M
Nihon Naibunpi Gakkai Zasshi. 1979 Jun 20;55(6):797-804. doi: 10.1507/endocrine1927.55.6_797.
We extracted the immunoreactive luteinizing hormone-releasing hormone (IR-LHRH) from villous and plasma in the first trimester of pregnancy with 0.2N acetic acid and the florisil absorbtion method. Recovery rate was 40.0 +/- 4.08% in the former and 62.5 +/- 9.5% in the latter, respectively. These IR-LHRH were measured by radioimmunoassay, which showed sensitivity from 5 to 500 pg/ml. The villous IR-LHRH was compared with synthetic LH-RH by a dilution curve in different concentrations and the column gel filtration of sephadex-G25. The dilution curve of synthetic LH-RH and sample material showed a significant correlation, and the elution time of villous IR-LHRH on the column was something faster than that of synthetic LH-RH. The concentration of villous IR-LHRH showed 20--100 pg/0.1g dry weight in the first trimester of pregnancy, and the peak was at 9 weeks of normal pregnancy and at 8 weeks of inevitable abortion respectively, but these IR-LHRH levels were not changed between pre- and post-dilation and curettage. From this evidence, we considered that IR-LHRH show activity of the local hormone in villous tissue similar to somatostation.
我们采用0.2N乙酸及弗罗里硅土吸附法，从妊娠早期的绒毛和血浆中提取免疫反应性促黄体生成激素释放激素（IR-LHRH）。前者的回收率为40.0±4.08%，后者为62.5±9.5%。这些IR-LHRH通过放射免疫测定法进行检测，其灵敏度为5至500 pg/ml。通过不同浓度的稀释曲线以及葡聚糖凝胶G-25柱凝胶过滤，将绒毛IR-LHRH与合成LH-RH进行比较。合成LH-RH与样品材料的稀释曲线显示出显著相关性，且绒毛IR-LHRH在柱上的洗脱时间比合成LH-RH稍快。妊娠早期绒毛IR-LHRH的浓度为20 - 100 pg/0.1g干重，在正常妊娠9周和难免流产8周时达到峰值，但刮宫前后这些IR-LHRH水平无变化。基于此证据，我们认为IR-LHRH在绒毛组织中表现出类似于生长抑素的局部激素活性。
