酵母共激活因子MBF1介导GCN4依赖性转录激活。
Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation.
作者信息
Takemaru K, Harashima S, Ueda H, Hirose S
机构信息
Department of Developmental Genetics, National Institute of Genetics, and Department of Genetics, The Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411-8540, Japan.
出版信息
Mol Cell Biol. 1998 Sep;18(9):4971-6. doi: 10.1128/MCB.18.9.4971.
Abstract
Transcriptional coactivators play a crucial role in gene expression by communicating between regulatory factors and the basal transcription machinery. The coactivator multiprotein bridging factor 1 (MBF1) was originally identified as a bridging molecule that connects the Drosophila nuclear receptor FTZ-F1 and TATA-binding protein (TBP). The MBF1 sequence is highly conserved across species from Saccharomyces cerevisiae to human. Here we provide evidence acquired in vitro and in vivo that yeast MBF1 mediates GCN4-dependent transcriptional activation by bridging the DNA-binding region of GCN4 and TBP. These findings indicate that the coactivator MBF1 functions by recruiting TBP to promoters where DNA-binding regulators are bound.
摘要
转录共激活因子通过在调控因子和基础转录机制之间进行沟通，在基因表达中发挥关键作用。共激活因子多蛋白桥联因子1（MBF1）最初被鉴定为连接果蝇核受体FTZ-F1和TATA结合蛋白（TBP）的桥联分子。从酿酒酵母到人类，MBF1序列在物种间高度保守。在此，我们提供了体内和体外获得的证据，表明酵母MBF1通过桥联GCN4的DNA结合区域和TBP来介导GCN4依赖性转录激活。这些发现表明，共激活因子MBF1通过将TBP招募到与DNA结合调节因子结合的启动子上发挥作用。
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