Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, South Korea.
Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, South Korea.
Virus Res. 2023 Mar;326:199061. doi: 10.1016/j.virusres.2023.199061. Epub 2023 Feb 8.
Fusarium graminearum virus 2 (FgV2) infection induces phenotypic changes like reduction of growth rate and virulence with an alteration of the transcriptome, including various transcription factor (TFs) gene transcripts in Fusarium graminearum. Transcription factors are the primary regulator in many cellular processes and are significant in virus-host interactions. However, a detailed study about specific TFs to understand interactions between FgV2 and F. graminearum has yet to be conducted. We transferred FgV2 to a F. graminearum TF gene deletion mutant library to identify host TFs related to FgV2 infection. FgV2-infected TF mutants were classified into three groups depending on colony growth. The FgV2 accumulation level was generally higher in TF mutants showing more reduced growth. Among these FgV2-infected TF mutants, we found several possible TFs that might be involved in FgV2 accumulation, generation of defective interfering RNAs, and transcriptional regulation of FgDICER-2 and FgAGO-1 in response to virus infection. We also investigated the relation between FgV2 accumulation and production of reactive oxygen species (ROS) and DNA damage in fungal host cells by using DNA damage- or ROS-responsive TF deletion mutants. Our studies provide insights into the host factors related to FgV2 infection and bases for further investigation to understand interactions between FgV2 and F. graminearum.
尖孢镰刀菌病毒 2（FgV2）感染会引起表型变化，例如生长速度和毒力降低，同时转录组发生改变，包括尖孢镰刀菌中的各种转录因子（TF）基因转录本。转录因子是许多细胞过程的主要调节剂，在病毒-宿主相互作用中具有重要意义。然而，关于特定 TF 的详细研究，以了解 FgV2 和 F. graminearum 之间的相互作用尚未进行。我们将 FgV2 转移到尖孢镰刀菌 TF 基因缺失突变体文库中，以鉴定与 FgV2 感染相关的宿主 TF。根据菌落生长情况，将感染 FgV2 的 TF 突变体分为三组。在生长受抑制更严重的 TF 突变体中，FgV2 的积累水平通常更高。在这些感染 FgV2 的 TF 突变体中，我们发现了几个可能参与 FgV2 积累、产生缺陷干扰 RNA 和转录调控 FgDICER-2 和 FgAGO-1 以响应病毒感染的 TF。我们还通过使用 DNA 损伤或 ROS 反应性 TF 缺失突变体，研究了 FgV2 积累与真菌宿主细胞中活性氧（ROS）和 DNA 损伤之间的关系。我们的研究为与 FgV2 感染相关的宿主因子提供了深入了解，并为进一步研究 FgV2 和 F. graminearum 之间的相互作用提供了基础。
