一种含有修饰染色质结构因子的人类RNA聚合酶II复合物。
A human RNA polymerase II complex containing factors that modify chromatin structure.
作者信息
Cho H, Orphanides G, Sun X, Yang X J, Ogryzko V, Lees E, Nakatani Y, Reinberg D
机构信息
Howard Hughes Medical Institute, Division of Nucleic Acid Enzymology, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA.
出版信息
Mol Cell Biol. 1998 Sep;18(9):5355-63. doi: 10.1128/MCB.18.9.5355.
Abstract
We have isolated a human RNA polymerase II complex that contains chromatin structure remodeling activity and histone acetyltransferase activity. This complex contains the Srb proteins, the Swi-Snf complex, and the histone acetyltransferases CBP and PCAF in addition to RNA polymerase II. Notably, the general transcription factors are absent from this complex. The complex was purified by two different methods: conventional chromatography and affinity chromatography using antibodies directed against CDK8, the human homolog of the yeast Srb10 protein. Protein interaction studies demonstrate a direct interaction between RNA polymerase II and the histone acetyltransferases p300 and PCAF. Importantly, p300 interacts specifically with the nonphosphorylated, initiation-competent form of RNA polymerase II. In contrast, PCAF interacts with the elongation-competent, phosphorylated form of RNA polymerase II.
摘要
我们分离出了一种人类RNA聚合酶II复合物，它具有染色质结构重塑活性和组蛋白乙酰转移酶活性。除RNA聚合酶II外，该复合物还包含Srb蛋白、Swi-Snf复合物以及组蛋白乙酰转移酶CBP和PCAF。值得注意的是，该复合物中不存在通用转录因子。该复合物通过两种不同方法纯化：传统色谱法和使用针对酵母Srb10蛋白的人类同源物CDK8的抗体进行亲和色谱法。蛋白质相互作用研究表明RNA聚合酶II与组蛋白乙酰转移酶p300和PCAF之间存在直接相互作用。重要的是，p300与非磷酸化的、具有起始能力的RNA聚合酶II形式特异性相互作用。相比之下，PCAF与具有延伸能力的、磷酸化形式的RNA聚合酶II相互作用。
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本文引用的文献
1
The p300/CBP family: integrating signals with transcription factors and chromatin.p300/CBP 家族：将信号与转录因子和染色质整合在一起。
Trends Cell Biol. 1997 Jun;7(6):230-6. doi: 10.1016/S0962-8924(97)01048-9.
2
NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription.NAT是一种含有Srb多肽的人类复合物，其作为活化转录的负调节因子发挥作用。
Mol Cell. 1998 Aug;2(2):213-22. doi: 10.1016/s1097-2765(00)80131-8.
3
Molecular genetics of the RNA polymerase II general transcriptional machinery.RNA聚合酶II通用转录机制的分子遗传学
Microbiol Mol Biol Rev. 1998 Jun;62(2):465-503. doi: 10.1128/MMBR.62.2.465-503.1998.
4
FACT, a factor that facilitates transcript elongation through nucleosomes.FACT，一种促进转录本通过核小体进行延伸的因子。
Cell. 1998 Jan 9;92(1):105-16. doi: 10.1016/s0092-8674(00)80903-4.
5
Factors associated with the mammalian RNA polymerase II holoenzyme.与哺乳动物RNA聚合酶II全酶相关的因素。
Nucleic Acids Res. 1998 Feb 1;26(3):847-53. doi: 10.1093/nar/26.3.847.
6
Genetics of transcriptional regulation in yeast: connections to the RNA polymerase II CTD.酵母转录调控的遗传学：与RNA聚合酶II CTD的联系
Annu Rev Cell Dev Biol. 1997;13:1-23. doi: 10.1146/annurev.cellbio.13.1.1.
7
NF-Y is associated with the histone acetyltransferases GCN5 and P/CAF.核因子Y与组蛋白乙酰转移酶GCN5和P/CAF相关。
J Biol Chem. 1998 Jan 16;273(3):1430-4. doi: 10.1074/jbc.273.3.1430.
8
The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain.酵母的中介蛋白及其通过RNA聚合酶II羧基末端结构域的功能。
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9
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Genes Dev. 1997 Dec 15;11(24):3279-85. doi: 10.1101/gad.11.24.3279.
10
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Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):12758-60. doi: 10.1073/pnas.94.24.12758.
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