采用不连续聚丙烯酰胺凝胶电泳法检测日本人群中的两个短串联重复序列位点（HumTPO和HumLPL）。

Detection of the two short tandem repeat loci (HumTPO and HumLPL) in Japanese populations using discontinuous polyacrylamide gel electrophoresis.

作者信息

Takeshita H, Yasuda T, Nakajima T, Hosomi O, Nakashima Y, Tsutsumi S, Kishi K

机构信息

Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Japan.

出版信息

Nihon Hoigaku Zasshi. 1998 Apr;52(2):139-43.

PMID:9711065

Abstract

Two short tandem repeat (STR) systems, HumTPO and HumLPL, were investigated in 218 unrelated Japanese living in Gunma and Fukui Prefectures by means of discontinuous, horizontal polyacrylamide gel electrophoresis and silver staining. Five and six alleles were identified at the HumTPO and HumLPL loci, respectively. No deviations from the Hardy-Weinberg equilibrium or differences in genotype frequency were found between the two Japanese populations in the two systems. Our detection methods offered several advantages including time-saving and safe electrophoretic procedures, and fast and easy silver-staining, in comparison with standard and conventional STR analysis using vertical polyacrylamide gel electrophoresis.

摘要

通过不连续水平聚丙烯酰胺凝胶电泳和银染法，对居住在群马县和福井县的218名无血缘关系的日本人，研究了两个短串联重复序列（STR）系统，即HumTPO和HumLPL。在HumTPO和HumLPL基因座上分别鉴定出5个和6个等位基因。在这两个系统中，两个日本人群之间均未发现偏离哈迪-温伯格平衡或基因型频率存在差异。与使用垂直聚丙烯酰胺凝胶电泳的标准和传统STR分析相比，我们的检测方法具有诸多优势，包括省时且安全的电泳程序，以及快速简便的银染法。