Thibaudeau G, Holder S, Gerard P
Department of Biological Sciences, Mississippi State University, Mississippi State.
Pigment Cell Res. 1998 Aug;11(4):189-97. doi: 10.1111/j.1600-0749.1998.tb00729.x.
The neural crest of vertebrate embryos has been used to elucidate steps involved in early embryonic cellular processes such as differentiation and migration. Neural crest cells form a ridge along the dorsal midline and subsequently they migrate throughout the embryo and differentiate into a wide variety of cell types. Intrinsic factors and environmental cues distributed along the neural tube, along the migratory pathways, and/or at the location of arrest influence the fate of neural crest cells. Although premigratory cells of the cranial and trunk neural crest exhibit differences in their differentiation potentials, premigratory trunk neural crest cells are generally assumed to have equivalent developmental potentials. Axolotl neural crest cells from different regions of origin, different stages of development, and challenged with different culture media have been analyzed for differentiation preferences pertaining to the pigment cell lineages. We report region-dependent differentiation of chromatophores from trunk neural crest at two developmental stages. Also, dosage with guanosine produces region-specific influences on the production of xanthophores from wild-type embryos. Our results support the hypothesis that spatial and temporal differences among premigratory trunk neural crest cells found along the anteroposterior axis influence developmental potentials and diminish the equivalency of axolotl neural crest cells.
脊椎动物胚胎的神经嵴已被用于阐明早期胚胎细胞过程（如分化和迁移）中涉及的步骤。神经嵴细胞沿着背中线形成一条嵴，随后它们迁移到整个胚胎中并分化为多种细胞类型。沿着神经管、沿着迁移途径和/或在停滞位置分布的内在因素和环境线索影响神经嵴细胞的命运。尽管颅神经嵴和躯干神经嵴的迁移前细胞在分化潜能上存在差异，但一般认为迁移前的躯干神经嵴细胞具有同等的发育潜能。对来自不同起源区域、不同发育阶段并受到不同培养基挑战的蝾螈神经嵴细胞进行了分析，以确定其在色素细胞谱系方面的分化偏好。我们报告了在两个发育阶段躯干神经嵴色素细胞的区域依赖性分化。此外，用鸟苷处理对野生型胚胎黄色素细胞的产生具有区域特异性影响。我们的结果支持这样的假设，即沿前后轴发现的迁移前躯干神经嵴细胞之间的时空差异会影响发育潜能，并降低蝾螈神经嵴细胞的等同性。
