Moynier M, Kavsan V, Gales C, Montagnier L, Bahraoui E
Laboratoire d'Immuno-virologie, Université Paul Sabatier, Toulouse, France.
Vaccine. 1998 Oct;16(16):1523-30. doi: 10.1016/s0264-410x(98)00036-x.
Mice immunized with plasmid DNA encoding Nef accessory protein of human immunodeficiency virus type 1 developed high levels of anti-Nef antibodies which were maintained for at least 16 months. These antibodies produced in response to Nef-expressing plasmid DNA did not recognize the linear peptides except the long C-terminal peptide for three of the ten sera. With anti-Nef antibodies produced in mice immunized with the protein Nef without any adjuvant, the same restraint epitope binding was found. On the contrary, anti-Nef antibodies from mice immunized with the protein in Freund's adjuvant showed a broader epitope reactivity pattern. Interestingly, the analysis of immunoglobulin isotype profiles of antibodies generated by the different protocols of immunization showed that plasmid DNA immunization induced predominantly IgG2a, whereas immunization with Nef protein, with or without adjuvant, yielded a preponderance of IgG1 antibodies.
用编码1型人类免疫缺陷病毒Nef辅助蛋白的质粒DNA免疫的小鼠产生了高水平的抗Nef抗体，这些抗体至少维持了16个月。在十份血清中的三份中，这些针对表达Nef的质粒DNA产生的抗体除了长的C末端肽外，不识别线性肽。在用无任何佐剂的Nef蛋白免疫的小鼠中产生的抗Nef抗体，也发现了相同的限制性表位结合情况。相反，在用弗氏佐剂中的蛋白免疫的小鼠产生的抗Nef抗体显示出更广泛的表位反应模式。有趣的是，对不同免疫方案产生的抗体的免疫球蛋白同种型谱分析表明，质粒DNA免疫主要诱导IgG2a，而用Nef蛋白免疫（无论有无佐剂）则产生大量的IgG1抗体。
