在呼吸的酿酒酵母细胞中，乙酰辅酶A合成酶同工酶的过量产生不会降低暴露于葡萄糖过量环境后的乙酸盐产量。
Overproduction of acetyl-coenzyme A synthetase isoenzymes in respiring Saccharomyces cerevisiae cells does not reduce acetate production after exposure to glucose excess.
作者信息
de Jong-Gubbels P, van den Berg M A, Luttik M A, Steensma H Y, van Dijken J P, Pronk J T
机构信息
Department of Microbiology and Enzymology, Kluyver Institute of Biotechnology, Delft University of Technology, The Netherlands.
出版信息
FEMS Microbiol Lett. 1998 Aug 1;165(1):15-20. doi: 10.1111/j.1574-6968.1998.tb13121.x.
To investigate whether the production of acetate which occurs after exposure of respiring Saccharomyces cerevisiae cells to excess glucose can be reduced by overproduction of acetyl-CoA synthetase (ACS, EC 6.2.1.1), the ACS1 and ACS2 genes were introduced on multi-copy plasmids. For each isoenzyme, the level in glucose-limited chemostat cultures was increased by 3-6-fold, relative to an isogenic reference strain. However, ACS overproduction did not result in a reduced production of acetate after a glucose pulse (100 mmol l-1) to these cultures. This indicates that a limited capacity of ACS is not the sole cause of acetate accumulation in S. cerevisiae.
为了研究在呼吸的酿酒酵母细胞暴露于过量葡萄糖后产生的乙酸盐产量是否可以通过过量表达乙酰辅酶A合成酶（ACS，EC 6.2.1.1）来降低，将ACS1和ACS2基因导入多拷贝质粒。对于每种同工酶，相对于同基因参考菌株，葡萄糖限制恒化器培养物中的水平提高了3至6倍。然而，在向这些培养物中加入葡萄糖脉冲（100 mmol l-1）后，ACS的过量表达并未导致乙酸盐产量降低。这表明ACS能力有限不是酿酒酵母中乙酸盐积累的唯一原因。
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