Chiodera P, Volpi R, Coiro V
Department of Internal Medicine, School of Medicine, University of Parma, Italy.
Metabolism. 1998 Aug;47(8):897-9. doi: 10.1016/s0026-0495(98)90340-7.
To establish whether nitric-oxide (NO) participates in the regulation of prolactin (PRL) secretion in humans in basal conditions and/or under stimulation with vasoactive intestinal polypeptide (VIP), seven normal men were treated with a placebo (normal saline) or the NO synthase (NOS) inhibitor L-NAME (40 microg/kg injected plus 50 microg/kg infused intravenously over 60 minutes), which in previous studies has been found able to modify other pituitary hormone secretions. Experiments were performed either in basal conditions or during stimulation of PRL secretion with an intravenous infusion of VIP (4 pmol/kg min over 60 minutes). The administration of L-NAME was unable to change the basal secretion of PRL. In contrast, L-NAME significantly enhanced the PRL increase induced by VIP. These data argue against an involvement of NO in regulation of basal PRL secretion. In contrast, the stimulatory effect of L-NAME on VIP-induced PRL secretion suggests that NO exerts an inhibitory control of the PRL response to VIP.
为确定在基础状态下和/或在用血管活性肠肽（VIP）刺激时，一氧化氮（NO）是否参与人体催乳素（PRL）分泌的调节，对7名正常男性用安慰剂（生理盐水）或NO合酶（NOS）抑制剂L-精氨酸甲酯（L-NAME，静脉注射40微克/千克，再在60分钟内静脉输注50微克/千克）进行治疗，在先前的研究中已发现该抑制剂能够改变其他垂体激素的分泌。实验在基础状态下进行，或在用VIP静脉输注（60分钟内4皮摩尔/千克·分钟）刺激PRL分泌期间进行。给予L-NAME未能改变PRL的基础分泌。相反，L-NAME显著增强了VIP诱导的PRL升高。这些数据表明NO不参与基础PRL分泌的调节。相反，L-NAME对VIP诱导的PRL分泌的刺激作用表明，NO对PRL对VIP的反应发挥抑制性控制作用。
