Volpi R, Chiodera P, Caffarri G, Vescovi P P, Capretti L, Gatti C, Coiro V
Department of Internal Medicine, School of Medicine, University of Parma, Italy.
Neuropeptides. 1996 Dec;30(6):528-32. doi: 10.1016/s0143-4179(96)90034-7.
In order to establish whether nitric oxide (NO) is involved in the regulation of ACTH and/or GH secretion, normal male subjects were treated i.v. with the NO-synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) (40 micrograms/kg injected plus 50 micrograms/kg infused over 60 min) in basal conditions and/or during stimulation with insulin (0.15 IU/kg body weight in an i.v. bolus) to induce hypoglycemia (ITT) or ASP 1 ILE-5 angiotensin II (ANG II) (increasing doses of 4, 8 and 16 ng/kg/min, each dose for 20 min). The administration of L-NAME neither changed the basal secretion of ACTH and GH nor modified the hormonal responses to ANG II stimulation. Also the GH response during ITT remained unchanged in the presence of L-NAME. In contrast, the ACTH response to hypoglycemia was significantly higher when L-NAME was administered. These data suggest that in normal men NO has a negative effect on ACTH secretion, but not GH secretion, in response to hypoglycemia. Furthermore, our results argue against a role of NO in the control of basal and ANG II-stimulated ACTH and GH secretions.
为了确定一氧化氮（NO）是否参与促肾上腺皮质激素（ACTH）和/或生长激素（GH）分泌的调节，对正常男性受试者在基础状态下和/或在用胰岛素（静脉推注0.15 IU/kg体重）诱导低血糖（胰岛素耐量试验，ITT）或静脉输注1-异亮氨酸-5-血管紧张素II（ANG II）（递增剂量为4、8和16 ng/kg/min，每个剂量持续20分钟）进行刺激期间，静脉注射一氧化氮合酶（NOS）抑制剂NG-硝基-L-精氨酸甲酯（L-NAME）（先静脉注射40微克/千克，再在60分钟内静脉输注50微克/千克）。给予L-NAME既未改变ACTH和GH的基础分泌，也未改变对ANG II刺激的激素反应。在L-NAME存在的情况下，ITT期间的GH反应也保持不变。相反，给予L-NAME时，对低血糖的ACTH反应显著更高。这些数据表明，在正常男性中，NO对低血糖反应时的ACTH分泌有负面影响，但对GH分泌没有影响。此外，我们的结果表明NO在基础状态和ANG II刺激的ACTH和GH分泌控制中不起作用。
