Biksi I, Kacskovics I, Mándoki M, Iván J, Horváth-Papp I, Makay G, Vetési F
Department of Pathology and Forensic Veterinary Medicine, University of Veterinary Science, Budapest, Hungary.
Acta Vet Hung. 1998;46(4):415-20.
Pooled faecal samples and/or intestinal contents from pig carcasses were collected from 11 Hungarian swine farms and subjected to DNA extraction/ purification and subsequent polymerase chain reaction (PCR) in order to detect Lawsonia intracellularis, the aetiological agent of the porcine proliferative enteropathy complex. Specific PCR positivity was detected in 6 individual and 22 pooled samples out of 46, originating from eight herds. The PCR products of collected faecal samples hybridised in Southern blot hybridisation with the DNA of the type strain L. intracellularis NCTC 12657. This is the first confirmed detection of L. intracellularis by PCR in Hungary.
从11个匈牙利养猪场收集猪尸体的混合粪便样本和/或肠道内容物，进行DNA提取/纯化以及随后的聚合酶链反应（PCR），以检测猪增生性肠病综合征的病原体胞内劳森菌。在来自8个猪群的46个样本中，6个个体样本和22个混合样本检测到特异性PCR阳性。收集的粪便样本的PCR产物在Southern印迹杂交中与胞内劳森菌模式菌株NCTC 12657的DNA杂交。这是匈牙利首次通过PCR确诊检测到胞内劳森菌。
