Barek J, Cvacka J, de Méo M, Laget M, Michelon J, Castegnaro M
Department of Analytical Chemistry, Charles University, Prague, Czech Republic.
Ann Occup Hyg. 1998 May;42(4):259-66. doi: 10.1016/s0003-4878(98)00023-4.
As a part of a program devoted to the destruction of antineoplastic agents, three chemical methods readily available in the hospital environment, viz. oxidation with sodium hypochlorite (NaClO, 5%), hydrogen peroxide (H2O2, 30%), and Fenton reagent (FeCl2.2H2O; 0.3 g in 10 ml H2O2, 30%), were tested for the degradation of four anticancer drugs: Amsacrine, Azathioprine, Asparaginase and Thiotepa. The efficiency of the degradation was monitored by high-performance liquid chromatography. The mutagenicity of the degradation residues were tested by Ames test using tester strains Salmonella typhimurium TA 97a, TA 98, TA 100 and TA 102 with and without an exogenous metabolic activation system. Using sodium hypochlorite, 98.5% of Amsacrine, 99.0% of Azathioprine, 99.5% of Asparaginase and 98.7% of Thiotepa were destroyed after 1 hr. The hydrogen peroxide treatment destroyed 99% of Asparaginase and 98.7% of Thiotepa in 1 hr. However, this procedure was not efficient for the treatment of Amsacrine (28% after 16 hr) and of Azathioprine (53% degradation in 4 hr). The action of Fenton reagent resulted in the destruction of 98% of Amsacrine, and 99.5% of Azathioprine, 98.5% of Asparaginase and 98.7% of Thiotepa in 1 hr. In all cases where a high degree of degradation was achieved, the residues obtained weee non mutagenic.
作为致力于抗肿瘤药物销毁项目的一部分，对医院环境中三种易于获取的化学方法，即使用次氯酸钠（NaClO，5%）、过氧化氢（H₂O₂，30%）和芬顿试剂（FeCl₂·2H₂O；0.3 g溶于10 ml 30%的H₂O₂中），测试了其对四种抗癌药物：安吖啶、硫唑嘌呤、天冬酰胺酶和塞替派的降解效果。通过高效液相色谱法监测降解效率。使用鼠伤寒沙门氏菌TA 97a、TA 98、TA 100和TA 102测试菌株，在有无外源性代谢激活系统的情况下，通过艾姆斯试验检测降解残留物的致突变性。使用次氯酸钠处理1小时后，98.5%的安吖啶、99.0%的硫唑嘌呤、99.5%的天冬酰胺酶和98.7%的塞替派被销毁。过氧化氢处理1小时后，99%的天冬酰胺酶和98.7%的塞替派被销毁。然而，该方法对安吖啶（16小时后为28%）和硫唑嘌呤（4小时内降解53%）的处理效果不佳。芬顿试剂作用1小时后，98%的安吖啶、99.5%的硫唑嘌呤、98.5%的天冬酰胺酶和98.7%的塞替派被销毁。在所有实现高度降解的情况下，所得残留物均无致突变性。
