Nishizuka S, Tamura G, Terashima M, Satodate R
Department of Pathology, School of Medicine, Iwate Medical University, Morioka, Japan.
J Pathol. 1998 May;185(1):38-43. doi: 10.1002/(SICI)1096-9896(199805)185:1<38::AID-PATH58>3.0.CO;2-T.
In a recent allelotypic analysis of differentiated adenocarcinoma of the stomach, loss of heterozygosity (LOH) was found frequently on chromosomes 2q, 4p, 5q, 6p, 11q, 14q, 17p, 18q, and 21q. To clarify the sequence of these chromosomal losses during gastric carcinogenesis, microsatellite analysis of the chromosome arms described above was performed in 25 early and 29 advanced differentiated adenocarcinomas of the stomach. LOH on these chromosome arms fell within a range of 20-50 per cent. On 4p, 7q, 14q, 17p, and 21q, LOH was detected at a similar frequency in both early and advanced carcinomas, while LOH on 2q, 5q, 6p, 11q, and 18q was observed more than twice as frequently in advanced than in early lesions. Mean fractional allelic losses (FALs) were 0.221 in early and 0.413 in advanced carcinomas, representing a significant difference P < 0.05). These results suggest that LOH on 4p, 7q, 14q, 17p, and 21q is a relatively early event, while LOH on 2q, 5q, 6p, 11q, and 18q typically accumulates during the progression of gastric carcinogenesis.
在最近一项关于胃分化腺癌的等位基因分型分析中，发现2号染色体长臂、4号染色体短臂、5号染色体长臂、6号染色体短臂、11号染色体长臂、14号染色体长臂、17号染色体短臂、18号染色体长臂和21号染色体长臂上经常出现杂合性缺失（LOH）。为了阐明胃癌发生过程中这些染色体缺失的顺序，对25例早期和29例晚期胃分化腺癌进行了上述染色体臂的微卫星分析。这些染色体臂上的LOH发生率在20%至50%之间。在4号染色体短臂、7号染色体长臂、14号染色体长臂、17号染色体短臂和21号染色体长臂上，早期和晚期癌中LOH的检出频率相似，而在2号染色体长臂、5号染色体长臂、6号染色体短臂、11号染色体长臂和18号染色体长臂上，晚期病变中LOH的检出频率是早期病变的两倍多。早期癌的平均等位基因缺失分数（FAL）为0.221，晚期癌为0.413，差异有统计学意义（P<0.05）。这些结果表明，4号染色体短臂、7号染色体长臂、14号染色体长臂、17号染色体短臂和21号染色体长臂上的LOH是相对早期事件，而2号染色体长臂、5号染色体长臂、6号染色体短臂、11号染色体长臂和18号染色体长臂上的LOH通常在胃癌发生进展过程中积累。
