Eremin A N, Metelitsa D I
Prikl Biokhim Mikrobiol. 1994 Mar-Apr;30(3):340-8.
Catalase was immobilized on periodate-activated cellulose membranes in a medium with the bicarbonate buffer and in reversed micelles of Aerosol OT in heptane. The enzyme effectively bound with the carriers in micellar media within a wide range of initial concentrations. The efficiency of immobilization depended on the volume of the polar phase in heptane, pH, and molarity of the bicarbonate buffer in reversed micelles, as well as on the process duration. The catalase covalently immobilized on cellulose membranes in reversed micelles was ten times more active compared to that immobilized on the same carrier in aqueous medium. Reversed micelles stabilized catalase at low concentrations of the enzyme in contact solution and hindered its inactivation on the carrier surface.
过氧化氢酶在含有碳酸氢盐缓冲液的介质中以及在庚烷中气溶胶OT的反胶束中固定在高碘酸盐活化的纤维素膜上。在广泛的初始浓度范围内，该酶能有效地与胶束介质中的载体结合。固定化效率取决于庚烷中极性相的体积、反胶束中碳酸氢盐缓冲液的pH值和摩尔浓度以及固定化过程的持续时间。与在水介质中固定在相同载体上的过氧化氢酶相比，在反胶束中固定在纤维素膜上的过氧化氢酶活性高十倍。反胶束在接触溶液中低浓度酶的情况下稳定过氧化氢酶，并阻碍其在载体表面失活。
