从菠菜叶绿体中分离并鉴定6-羟甲基蝶呤作为克氏锥虫生长促进因子。
Isolation and characterization of 6-hydroxymethylpterin as the Crithidia growth-promoting factor from spinach chloroplasts.
作者信息
Iwai K, Bunno M, Kobashi M, Suzuki T
出版信息
Biochim Biophys Acta. 1976 Sep 24;444(2):618-22. doi: 10.1016/0304-4165(76)90406-2.
PMID:971422
Abstract
2-Amino-4-hydroxypteridine compounds were isolated as the Crithidia factor from spinach chloroplasts by DEAE-Sephadex A-50, DEAE-cellulose, Sephadex G-25, and thin-layer chromatography. One of the compounds was characterized as 6-hydroxymethylpterin by gas-liquid chromatography-mass spectrometry and by comparison with authentic specimen.
摘要
通过二乙氨基乙基葡聚糖A-50、二乙氨基乙基纤维素、葡聚糖G-25和薄层色谱法从菠菜叶绿体中分离出2-氨基-4-羟基蝶啶化合物作为克氏锥虫因子。其中一种化合物通过气-液色谱-质谱联用并与标准样品比较，被鉴定为6-羟甲基蝶呤。
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