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从菠菜叶绿体中分离并鉴定6-羟甲基蝶呤作为克氏锥虫生长促进因子。

Isolation and characterization of 6-hydroxymethylpterin as the Crithidia growth-promoting factor from spinach chloroplasts.

作者信息

Iwai K, Bunno M, Kobashi M, Suzuki T

出版信息

Biochim Biophys Acta. 1976 Sep 24;444(2):618-22. doi: 10.1016/0304-4165(76)90406-2.

DOI:10.1016/0304-4165(76)90406-2
PMID:971422
Abstract

2-Amino-4-hydroxypteridine compounds were isolated as the Crithidia factor from spinach chloroplasts by DEAE-Sephadex A-50, DEAE-cellulose, Sephadex G-25, and thin-layer chromatography. One of the compounds was characterized as 6-hydroxymethylpterin by gas-liquid chromatography-mass spectrometry and by comparison with authentic specimen.

摘要

通过二乙氨基乙基葡聚糖A-50、二乙氨基乙基纤维素、葡聚糖G-25和薄层色谱法从菠菜叶绿体中分离出2-氨基-4-羟基蝶啶化合物作为克氏锥虫因子。其中一种化合物通过气-液色谱-质谱联用并与标准样品比较,被鉴定为6-羟甲基蝶呤。

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Isolation and characterization of 6-hydroxymethylpterin as the Crithidia growth-promoting factor from spinach chloroplasts.从菠菜叶绿体中分离并鉴定6-羟甲基蝶呤作为克氏锥虫生长促进因子。
Biochim Biophys Acta. 1976 Sep 24;444(2):618-22. doi: 10.1016/0304-4165(76)90406-2.
2
Isolation and characterization of 6-hydroxymethylpterin as a Crithidia growth-promoting factor from spinach chloroplasts.从菠菜叶绿体中分离并鉴定6-羟甲基蝶呤作为克氏锥虫生长促进因子
Methods Enzymol. 1980;66:512-7. doi: 10.1016/0076-6879(80)66495-7.
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6-Methyltetrahydropterin. Isolation and identification as the highly active hydroxylase cofactor from tetrahydrofolate.6-甲基四氢蝶呤。从四氢叶酸中分离并鉴定为高活性羟化酶辅因子。
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Folate and pterin metabolism by cancer cells in culture.培养的癌细胞中的叶酸和蝶呤代谢
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引用本文的文献

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Folate biosynthesis in higher plants. cDNA cloning, heterologous expression, and characterization of dihydroneopterin aldolases.高等植物中的叶酸生物合成。二氢新蝶呤醛缩酶的cDNA克隆、异源表达及特性分析。
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