Meyer G, Hanon E, Georlette D, Pastoret P P, Thiry E
Virology-Immunology, Faculty of Veterinary Medicine, University of Liège, Belgium.
J Gen Virol. 1998 Aug;79 ( Pt 8):1983-7. doi: 10.1099/0022-1317-79-8-1983.
Bovine herpesvirus type 1 (BHV-1) glycoprotein H (gH) is a structural component of the virion which forms a complex with glycoprotein gL. To study the role of BHV-1 gH in the virus infectious cycle, a gH null mutant was constructed in which the gH coding sequences were deleted and replaced by the Escherichia coli lacZ cassette. The BHV-1 gH null mutant was propagated in trans-complementing MDBK cells, stably transfected with plasmid pMEP4 containing the BHV-1 gH gene under the control of the inducible mouse metallothionein promoter. Experiments with the BHV-1 gH null mutant showed that gH is essential in the infectious cycle of the virus and is specifically involved in virus entry and cell-to-cell spread. The lack of infectivity of virions devoid of gH is not due to a defect in attachment. Moreover, PEG-induced fusion of virions to target cells provides evidence that BHV-1 gH is required for virion penetration.
牛疱疹病毒1型（BHV-1）糖蛋白H（gH）是病毒粒子的一种结构成分，它与糖蛋白gL形成复合物。为了研究BHV-1 gH在病毒感染周期中的作用，构建了一个gH缺失突变体，其中gH编码序列被删除，并用大肠杆菌lacZ盒取代。BHV-1 gH缺失突变体在转互补MDBK细胞中繁殖，该细胞被稳定转染了质粒pMEP4，该质粒含有在可诱导的小鼠金属硫蛋白启动子控制下的BHV-1 gH基因。对BHV-1 gH缺失突变体的实验表明，gH在病毒的感染周期中至关重要，并且特别参与病毒进入和细胞间传播。缺乏gH的病毒粒子缺乏感染性并非由于附着缺陷。此外，聚乙二醇诱导的病毒粒子与靶细胞的融合提供了证据，表明BHV-1 gH是病毒粒子穿透所必需的。
