Hideshima T, Yamauchi A, Kimizuka H
Biochim Biophys Acta. 1976 Sep 21;448(1):155-66. doi: 10.1016/0005-2736(76)90084-5.
The transports of tritiated ATP, ADP and AMP from the aqueous to scintillator phase with and without octadecylamine (or dodecyl guanidine) have been studied by the layered scintillation method and a theory suitable for an explanation of the results has been presented. (1) Transport processes were all expressed by the first order kinetics. (2) For the simple partitioning of ATP, the reciprocal of the rate constant of the backward permeation was linear with respect to the square of the partition coefficient. (3) For the transport of nucleotide with chemical reaction, the reciprocal of the rate constant of the backward permeation was linear against the overall partition coefficient of nucleotide. (4) A theory was presented on the basis of a general diffusion equation by assuming the two-film model with potential energy near the interface. (5) The theory could explain the dependences of the permeation rates on the partition coefficients. (6) From the finding that the ratio of the apparent diffusion coefficient in aqueous to scintillator phase was much smaller than unity, the occurrence of an energy barrier at interface was suggested. For the simple partitioning of ATP, the energy barrier was not significant.
采用分层闪烁法研究了在有和没有十八胺（或十二烷基胍）的情况下，氚标记的ATP、ADP和AMP从水相到闪烁体相的转运，并提出了一种适合解释该结果的理论。（1）转运过程均由一级动力学表示。（2）对于ATP的简单分配，反向渗透速率常数的倒数与分配系数的平方呈线性关系。（3）对于伴有化学反应的核苷酸转运，反向渗透速率常数的倒数与核苷酸的总分配系数呈线性关系。（4）在假设界面附近具有势能的双膜模型的基础上，基于一般扩散方程提出了一种理论。（5）该理论可以解释渗透速率对分配系数的依赖性。（6）从水相到闪烁体相的表观扩散系数之比远小于1这一发现，表明在界面处存在能垒。对于ATP的简单分配，能垒并不显著。
