Meng B, Pang S Z, Forsline P L, McFerson J R, Gonsalves D
Department of Plant Pathology, Cornell University, Geneva, NY 14456, USA.
J Gen Virol. 1998 Aug;79 ( Pt 8):2059-69. doi: 10.1099/0022-1317-79-8-2059.
Rupestris stem pitting (RSP), a component of the rugose wood complex, is one of the most widespread graft-transmissible diseases of grapevines. Here we report on the consistent association of a high molecular mass dsRNA (ca. 8.7 kbp) with RSP. The dsRNA was reverse-transcribed and cDNAs generated were cloned into Lambda ZAP II. Sequence analysis of the cDNA clones showed that the dsRNA was of viral origin and the putative virus was designated rupestris stem pitting associated virus-1 (RSPaV-1). The genome of RSPaV-1 consists of 8726 nt excluding a poly(A) tail at the 3' terminus. It has five potential open reading frames which have the capacity to code for the replicase (ORF1), the triple gene block (ORF2-4) and the coat protein (ORF5). Comparison of the genome structure and nucleotide and amino acid sequences indicated similarities of RSPaV-1 to apple stem pitting virus, and to a lesser extent, to potato virus M carlavirus. The possibility that different strains of RSPaV-1 or other viruses are associated with RSP is discussed.
岩生葡萄茎痘病（RSP）是皱木复合病的一个组成部分，是葡萄中最广泛传播的嫁接传染性病害之一。在此，我们报道一种高分子量双链RNA（约8.7 kbp）与RSP始终存在关联。该双链RNA被反转录，产生的cDNA被克隆到Lambda ZAP II中。对cDNA克隆的序列分析表明，该双链RNA源自病毒，这种推定的病毒被命名为岩生葡萄茎痘病相关病毒1（RSPaV-1）。RSPaV-1的基因组由8726个核苷酸组成，3'末端不包括多聚腺苷酸尾巴。它有五个潜在的开放阅读框，能够编码复制酶（ORF1）、三联基因块（ORF2 - 4）和外壳蛋白（ORF5）。基因组结构以及核苷酸和氨基酸序列的比较表明，RSPaV-1与苹果茎痘病毒相似，在较小程度上与马铃薯病毒M（香石竹潜隐病毒属）相似。文中还讨论了不同株系的RSPaV-1或其他病毒与RSP相关的可能性。
